By-product hydrosol of Eucalyptus globulus essential oil distillation as source of botanical insecticides: wealth from waste


  • Arun D. SHARMA Lyallpur Khalsa College, PG Department of Biotechnology, Jalandhar, Punjab (IN)
  • Inderjeet KAUR Lyallpur Khalsa College, PG Department of Biotechnology, Jalandhar, Punjab (IN)



botanicals; insecticide; plant allelochemicals; plant protection; toxicity


Worldwide use of chemical pesticides is on rise which is creating a big problem to environment and human health. Hence great interest has been generated to find out botanical leads having anti-pest properties from medicinal plants. The objective of this work was to evaluate the insecticidal potential of Eucalyptus globulus waste hydrosol obtained after essential oil distillation. Samples of Eucalyptus globulus genus were collected from near-by areas of study, and were hydro-distilled and their by-product ‘hydrosol was analyzed for phenolics and tannin determination. UV-VIS, FT-IR and fluorescent study was also conducted of by-product hydrosol. In addition, insecticidal activity of by-product hydrosol was also monitored against mealy bug. Substantial amount of phenolics and tannins were detected in the by-product hydrosol. UV and fluorescent spectroscopy revealed the presence of secondary metabolites. Significantly higher insecticidal activity was observed of by-product hydrosol against mealy bug. The results suggested that by-product hydrosol from Eucalyptus globulus essential oil distillation can be considered as potential candidates for bio-control of pests.


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Iori A, Grazioli D, Gentile E, Marano G, Salvatore G (2005). Acaricidal properties of the essential oil of Melaleuca alternifolia Cheel (tea tree oil) against nymphs of Ixodes ricinus. Veterinary Parasitology 129:173-176.

Isman MB (2000). Plant essential oils for pest and disease management. Crop Protection 19:603-608.

Jahn GC, Beardsley JW, González-Hernández H (2003). A review of the association of ants with mealybug wilt disease of pineapple. Proceedings of the Hawaiian Entomological Society 36:9-28.

Karpagasundari C, Kulothungan S (2014). Analysis of bioactive compounds in Physalis minima leaves using GC MS, HPLC, UV-VIS and FTIR techniques. Journal of Pharmacognosy and Phytochemistry 3:196-201.

Koul O, Walia S, Dhaliwal GS (2008). Essential oils as green pesticides: potential and constraints. Biopesticides International 4:63-84.

Lang M, Stober F, Lichtenthaler HK (2005). Fluorescence emission spectra of plant leaves and plant constituents. Radiation and Environmental Biophysics 1191(30):333-347.

Lee H (2004). Acaricidal activity of constituents identified in Foeniculum vulgare fruit oil against Dermatophagoides spp. (Acari: Pyroglyphidae). Journal of Agricultural and Food Chemistry 52:2887-2889.

Liu H-X, Sun S-Q, LvG-H, Chan KKC (2006). Study on Angelica and its different extracts by Fourier transform infrared spectroscopy and two-dimensional correlation IR spectroscopy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 64:321-326.

Mylle E, Codreanu MC, Boruc J, Russinova E (2013). Emission spectra profiling of fluorescent proteins in living plant cells. Plant Methods 9:1-8.

Pamo ET, Tendonkeng F, Kana JR, Payne VK, Boukila B, Lemoufouet J, ... Nanda AS (2005). A study of the acaricidal properties of an essential oil extracted from the leaves of Ageratum houstonianum. Veterinary Parasitology 128(3-4):319-323.

Parekh J, Chanda V (2007). In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turkish Journal of Biology 31:53‐58.

Paul KR, Irudayaraj V, Johnson M, Patric DR (2011). Phytochemical and anti-bacterial activity of epidermal glands extracts of Christella parasitica (L.) H. Lev. Asian Pacific Journal of Tropical Biomedicine 1(1):8-11.

Porto NM, Barros YLD, Basílio IJ, Agra MDF (2016). Microscopic and UV/Vis spectrophotometric characterization of Cissampelos pareira of Brazil and Africa. Revista Brasileira de Farmacognosia 26(2):135-146.

Rattan RS (2010). Mechanism of action of insecticidal secondary metabolites of plant origin. Crop Protection 29:913-920.

Sharma A, Pati PK (2013). First record of Ashwagandha as a new host to the invasive mealybug (Phenacoccus solenopsis Tinsley) in India. Entomological News 123:59-62.

Wall R (2007). Ectoparasites: future challenges in a changing world. Veterinary Parasitology 148:62-74.




How to Cite

SHARMA, A. D., & KAUR, I. . (2021). By-product hydrosol of Eucalyptus globulus essential oil distillation as source of botanical insecticides: wealth from waste . Notulae Scientia Biologicae, 13(1), 10854.



Research articles
DOI: 10.15835/nsb13110854