Explants selection for in vitro propagation of Pachyrhizus erosus L.

  • Idowu A. OBISESAN Bowen University Iwo, College of Agriculture, Engineering and Sciences, Pure and Applied Biology, Osun State
  • Ayobola M. A. SAKPERE Obafemi Awolowo University, Faculty of Science, Department of Botany, Ile-Ife, Osun State
  • Bamidele J. AMUJOYEGBE Obafemi Awolowo University, Faculty of Agriculture, Department of Crop production and Protection, Ile-Ife, Osun State
  • Michael S. AKINROPO Obafemi Awolowo University, Faculty of Science, Department of Botany, Ile-Ife, Osun State
Keywords: auxins; callus; contamination; cytokinins; legume; Pachyrhizus erosus; shoot regeneration


Pachyrhizus erosus tuber is rich in protein asides its agronomical value as a legume, but the seeds by which it is propagated have very low viability. This study established sterilization protocol and effect of various concentrations of auxins and cytokinins on callus production and shoot regeneration from explants of P. erosus. Explants and seeds were sterilized using sodiumhypochlorite (NaClO) solution (5, 10 and 15% v/v) for 5 and 10 mins. Nodal, stem and leaf explants from in vitro germinated P. erosus and tuber from field grown plant were sterilized and cultured on Murashige and Skoog (MS) medium (control) and MS combined with different concentrations of auxins (NAA and 2, 4-D) and cytokinin (BA and Kinetin) and the cultured explants were monitored in terms of degree of callus formation, morphology and colour of callus and also for shoot induction. The results showed that seeds of P. erosus sterilized with 10% NaClO solution for 10 mins and germinated in vitro is the best way of getting sterile nodal, stem and leaf explants for the in vitro propagation of the plant, while tuber explants could be sterilized with 15% NaClO for 10 minutes. Nodal explants inoculated in MS medium supplemented with 1.0 mg/L BA gave the highest shoot regeneration response, while stem explants inoculated on MS medium supplemented with 1.0 mg/L BA and 0.5 mg/L NAA also gave the highest amount of friable callus. The study concluded that in vitro germinated seeds were the best way of getting explant for P. erosus.


Metrics Loading ...


Ayisire BE, Ayisire ER, Akinbola PO (2012). Studies on Hollarrhena floribunda (G. Don) Durand and Schinz: Germination and seedling growth, and preliminary investigations on callus induction and plant regeneration. African Journal of Agricultural Research 7(47):6297-6304. https://doi.org/10.5897/AJAR12.1626

Bergthaller W, Dijkink BH, Langelaan HC, Vereijken JM (2001). Legume protein preparation and functional properties. Food 45(1):396-398. https://doi.org/10.1002/1521-3803(20011001)45:6<390::AID-FOOD390>3.0.CO;2-%23

Berkov S, Pavlov A, Georgiev V, Bastida J, Burrus M, Ilieva M, Codina C (2009). Alkaloid synthesis and accumulation in Leucojum aestivum in vitro cultures. Natural Product Communications 4(3):359-64. https://doi.org/10.1177/1934578X0900400328

Bunn E, Tan BH (2002). Microbial contaminants in plant tissue culture propagating. In: Sivasithamparam K, Dixon KW (Eds). Microorganisms in plant conservation and biodiversity. The Netherlands: Kluwer Academic Publishers, Dordrecht pp 307-335. https://link.springer.com/chapter/10.1007/0-306-48099-9_1

Cassells AC (1991). Problems in tissue culture: culture contamination. In: Debergh PC, Zimmerman RH (Eds). Micropropagation. Springer, Dordrecht https://doi.org/10.1007/978-94-009-2075-0_3

Chen ZL, Yan ZL, Xue H, Feng XD, Cao JY (2002). In vitro culture leaves and plantlet regeneration of Ziziphus jujube ‘Zhanhual Dongzao’. Plant Physiology Communique 38(6):584. http://en.cnki.com.cn/Article_en/CJFDTotal-ZWSL200206019.htm

Gupta RK, Tandon VR (2005). An experimental evaluation of anticonvulsant activity of Vitex negundo. Indian Journal of Physiology and Pharmacology 49:163-172. https://www.ijpp.com/IJPP%20archives/2005_49_2/199-205.pdf

He ZY, Wang YG, Shi WL, Yin MQ (2002). Tissue culture and plantlet regeneration of leaf of Ziziphus jujuba. Plant Physiology Communiques 38(5):457. https://link.springer.com/article/10.1007/s11240-017-1274-8

Kumar S, Chandra A (2009). Direct plant regeneration via multiple shoot induction in Stylosanthes seabrana. Cytologia 74(4):391-399. https://doi.org/10.1508/cytologia.74.391

Lochlainn NGK (2011). Genetic improvement of under utilized legumes. Ph.D. Thesis, University of Nottingham, Germany.

Mahna N, Vahed SZ, Khani S (2013) Plant in vitro culture goes nano: nanosilver-mediated decontamination of ex vitro explants. Journal of Nanomedical and Nanotechology 4:161. https://doi:10.4172/2157-7439.1000161

Marvasi M, Donnarumma F, Frandi A, Mastromei G, Sterflinger K, Tiano P, Perito B (2012). Black microcolonial fungi as deteriogens of two famous marble statues in Florence, Italy. International Biodeterioration & Biodegradation 68:36-44. https://doi.org/10.1016/j.ibiod.2011.10.011

Masood SB, Rizwana B (2010). Nutritional and functional properties of some promising legumes protein isolated. Pakistan Journal of Nutrition 9(4):373-379. http://pjbs.org/pjnonline/fin1693.pdf

Miransari M, Smith DL (2014). Plant hormones and seed germination. Environmental and Experimental Botany 99:110-121. https://doi.org/10.1016/j.envexpbot.2013.11.005

Miyazaki N, Nakagawa A, Iwasaki K (2013). Life cycle of phytoreoviruses visualized by electron microscopy and tomography. Frontier in Microbiology 4:306. https://doi.org/10.3389/fmicb.2013.00306

Monokesh KS, Shamima N, Shahedur R, Abu HMJ (2014). In vitro callus induction and plantlet regeneration of Achyranthes aspera L., a high value medicinal plant. Asian Pacific Journal of Tropical Biomedicine 4(1):40-46. https://doi.org/10.1016/S2221-1691(14)60206-9

Murashige T, Skoog F (1962). A revised medium from rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Naval MM, Llacer G, Badenes ML, Giordani E (2009). Adventitious shoot regeneration from leaf explants of the Persimmon (Diospyros kaki Thunb.) cv. ‘Rojo Brillante’. Acta Horticulturae 833:183-186. https://doi.org/10.17660/ActaHortic.2009.833.29

Neves LO, Tomaz L, Fevereiro MPS (2001). Micropropagation of Medicago truncatula Gaertn. cv. Jemalong and Medicago truncatula ssp. Narbonensis. Plant Cell, Tissue and Organ Culture 67:81-84. https://doi.org/10.1023/A:1011699608494

Niedz RP, Bausher MG (2002). Control of in vitro contamination of explants from greenhouse- and field-grown trees. In Vitro Cell Developmental Biology-Plant 38:468-471. https://doi.org/10.1079/IVP2002316

Nikam TD, Savanth RS, Parage RS (2008). Micropropagation of Ceropegia hirsute Wt. and Arn. A starchy tuberous asclepiad. Indian Journal of Biotechnology 7:129-132.http://nopr.niscair.res.in/handle/123456789/7331

Ogita S, Miyazaki J, Godo T, Kato Y (2009). Possibility for selective accumulation of polyphenolics in tissue cultures of Senno (Lychnis senno Sieboldet Zucc.). Natural Product Communications 4(3):377-380. https://doi.org/10.1177/1934578X0900400312

Ojiewo C, Keatinge DJ, Hughes J, Tenkouano A, Nair R, Varshney RM, … Silim S (2015). The role of vegetables and legumes in assuring food, nutrition and income security for vulnerable groups in Sub-Saharan Africa. World Medical and Health Policy 7(3):187-210. https://doi.org/10.1002/wmh3.148

Omamor IB, Asemota AO, Eke CR, Ezia EI (2007) Fungal contaminants of the oil palm tissue culture in Nigerian Institute for Oil Palm Research (NIFOR). African Journal of Agricultural Research 2(10):534-537. https://doi.org/10.5897/AJAR.9000361

Padonou SW, Hounyevou AK, Ahounou J, Houssou AJ, Fandohan P, … Koudande DO (2013) Yam bean (Pachyrhizus erosus) tuber processing in Benin: production and evaluation of the quality of yam bean-gari and yam bean-fortified gari. International Journal of Biological and Chemical Sciences 7(1):247-259. http://dx.doi.org/10.4314/ijbcs.v7ili.21

Rout GR (2005). Micropropagation of Clitoria ternatea Linn. (Fabaceae): an important medicinal plant. In Vitro Cellular & Developmental Biology. Plant 41(4):516-519. https://doi.org/10.1079/IVP2005675

Sakpere AM, Ayisire ER, Abioye OI (2011) Potential of Launea taraxacifolia (Willd) Ex. C. Jeffrey for in vitro Regeneration. Notulae Scientia Biologicae 3(3):93-96.

Santos ACO, Cavalcanti MSM, Coelho LCBB (1996). Chemical composition and nutritional potential of yam bean seeds (Pachyrizus erosus L. Urban). Plant Foods for Human Nutrition 49:35-41. https://doi.org/10.1007/BF01092520

SAS Institute Inc. SAS 9.1.2 (2004). Qualification Tools User’s Guide. SAS Institute Inc., Cary, NC USA.

Sathyanaraya BN, Verghese DB (2007) Plant tissue culture: practices and new experimental protocols. IK International Pvt Ltd.

Shagufta N, Ali A, Siddique FA, Iqbal J (2007). Multiple shoot formation from different explants of chick pea (Cicerarietinum L.). Pakistan Journal of Botany 39(6):2067-2073. https://216321532/links/0912f50a52ee2ce256000000.pdf

Shahinozzaman M, Azad MAK, Amin MN (2012). In vitro clonal propagation of a fast-growing legume tree-Acacia angium Willd. employing cotyledonary node explants. Notulae Scientia Biologicae 4(2):79-85. https://doi.org/10.15835/nsb427553

Shaik S, Dewir YH, Singh N, Nicholas A (2010). Micropropagation and bioreactor studies of the medicinally important plant Lessertia (Sutherlandia) frutescens L. South African Journal of Botany 76(2):180-186. https://doi.org/10.1016/j.sajb.2009.10.005

Singh RK, Anandhan S, García-Pérez LM, Ruiz-May E, Pérez EN, Quiroz-Figueroa FR (2019) An efficient protocol for in vitro propagation of the wild legume Cicer microphyllum Benth., a crop wild relative of chickpea (Cicer arietinum L.). In Vitro Cell Developmental Biology-Plant 55:9-14. https://doi.org/10.1007/s11627-018-09958-y

Singh V, Tyagi A, Chauhan PK, Kumari P, Kaushal S (2011) Identification and prevention of bacterial contamination on explant used in plant tissue culture labs. International Journal of Pharmacy and Pharmaceutical Sciences 3(4):160-163

Slavin JL (2005). Dietary fiber and body weight. Nutrition 21:411-418.

Smith M, Skjøth CA, Myszkowska DAU, Puc M, Stach A, … Brandt J (2008). Long-range transport of Ambrosia pollen to Poland. Agricultural and Forest Meteorology 148:1402-1411.

Sørensen M (1996). Yam bean (Pachyrhizus DC.). Promoting the conservation and use of underutilized and neglected crops. 2. Institute of Plant Genetics and Crop plant Research, Gatersleben/International Plant Genetic Resources Institute, Rome.

Srinivasan R, Yule S (2014). Combining bio-pesticides with chemical pesticides to manage legume pod borer (Maruca vitrata) on yard-long bean in Thailand. International Journal of Pest Management 60(1):67-72

How to Cite
OBISESAN, I. A., SAKPERE, A. M. A., AMUJOYEGBE, B. J., & AKINROPO, M. S. (2021). Explants selection for in vitro propagation of Pachyrhizus erosus L. Notulae Scientia Biologicae, 13(1), 10844. https://doi.org/10.15835/nsb13110844
Research articles
DOI: 10.15835/nsb13110844