Impact of essential oils of clove Syzygium aromaticum in rats exposed to stress by nickel

  • Djallal E. H. ADLI University of Dr. Moulay Tahar Saida, Faculty of Sciences, Biology Department, Laboratory of Biotoxicology, Pharmacognosy and Biological Valorization of plants (LBPVBP)
  • Karima GRELE University of Dr. Moulay Tahar Saida, Faculty of Sciences, Biology Department, Laboratory of Biotoxicology, Pharmacognosy and Biological Valorization of plants (LBPVBP)
  • Amine H. BORSALI University of Dr. Moulay Tahar Saida, Faculty of Sciences, Biology Department, Laboratory of Biotoxicology, Pharmacognosy and Biological Valorization of plants (LBPVBP)
  • Mostapha BRAHMI University of Dr. Moulay Tahar Saida, Faculty of Sciences, Biology Department, Laboratory of Biotoxicology, Pharmacognosy and Biological Valorization of plants (LBPVBP)
  • Wafaa ARABI University of Dr. Moulay Tahar Saida, Faculty of Sciences, Biology Department, Laboratory of Biotoxicology, Pharmacognosy and Biological Valorization of plants (LBPVBP)
  • Narimane TAIBI University of Dr. Moulay Tahar Saida, Faculty of Sciences, Biology Department, Laboratory of Biotoxicology, Pharmacognosy and Biological Valorization of plants (LBPVBP)
  • Miloud SLIMANI University of Dr. Moulay Tahar Saida, Faculty of Sciences, Biology Department, Laboratory of Biotoxicology, Pharmacognosy and Biological Valorization of plants (LBPVBP)
  • Khaled KAHLOULA University of Dr. Moulay Tahar Saida, Faculty of Sciences, Biology Department, Laboratory of Biotoxicology, Pharmacognosy and Biological Valorization of plants (LBPVBP)
Keywords: antioxidant activity; GC-MS; nickel; oxidative stress; Syzygium aromaticum

Abstract

The mechanisms that cause nickel (Ni) toxicity are multiple and potentially affect all cells in the body. For this purpose, we were interested in the oxidative stress induced by the Ni at the erythrocyte level at a dose of 2 g/l during the gestation and lactation period in the Wistar rats and the capacity of the clove essential oil, Syzygium aromaticum, (CEO) to restore or not this state of stress. Extraction of the CEO by hydro-distillation allowed us to obtain a CEO with a yield of 10.60% and the characterization of this essential oil by GC-MS indicates that the major components of this oil are: Eugenol (80.95%), eugenyl acetate (10.48%), β-caryophyllene (7.21%) and α-humulene (0.87%). The antioxidant activity of the CEO made in vitro showed a free radical scavenging capacity DPPH with an IC 50 of the order of 25.60 μg/ml. In addition, analysis of the erythrocyte antioxidant status indicated that Ni significantly increased the enzymatic activities of catalase (CAT) and superoxide dismutase (SOD) and significantly decreased the enzymatic activity of glutathione peroxidase (GPx), resulting in dysfunction of the antioxidant defense system. On the other hand, the administration of CEO by intraperitoneal (IP) over a period of 21 days to rats previously intoxicated with Ni, indicates that this CEO contributes significantly in improving defenses against free radical aggression, through a recovery. At the level of antioxidant enzyme activities by increasing their abilities to eliminate radical compounds.

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Published
2020-06-29
How to Cite
ADLI, D. E. H., GRELE, K., BORSALI, A. H., BRAHMI, M., ARABI, W., TAIBI, N., SLIMANI, M., & KAHLOULA, K. (2020). Impact of essential oils of clove Syzygium aromaticum in rats exposed to stress by nickel. Notulae Scientia Biologicae, 12(2), 233-241. https://doi.org/10.15835/nsb12210689
Section
Research articles