In vitro anti-urease, antioxidant, anticholinesterase, cytotoxic and in vivo anti-inflammatory potential of Satureja cuneifolia Ten.

  • Turgut TASKIN University of Marmara, Faculty of Pharmacy, 34668, Istanbul
  • Murat DOGAN University of Sivas Cumhuriyet, Faculty of Pharmacy, Sivas
  • Muhammet E. CAM University of Marmara, Faculty of Pharmacy, 34668, Istanbul; Center for Nanotechnology and Biomaterials Application and Research (NBUAM); University College London, Department of Mechanical Engineering, Torrington Place, London WC1E 7JE
  • Talip SAHIN University of Marmara, Faculty of Pharmacy, 34668, Istanbul
  • Ismail SENKARDES University of Marmara, Faculty of Pharmacy, 34668, Istanbul
Keywords: anti-urease; anticholinesterase; anti-inflammatory; cytotoxic; S. cuneifolia


Satureja cuneifolia Ten. (wild savoury) belongs to the Lamiaceae family and is used to produce essential oil and aromatic water. This plant is also used as a condiment and herbal tea due to its stimulating, tonic and carminative effects. The in vitro antioxidant, anti-urease, anticholinesterase and cytotoxic activities of the different extracts from the plant’s aerial parts were examined. Besides, the in vivo anti-inflammatory activities of the fraction and direct methanol extracts were determined comparatively. In the current study, fraction methanol extract exhibited the strongest ABTS (52.34 mM trolox/mg extract) radical scavenging and ferric reduction (17.22 mM Fe2+/mg extract) activity. It was also found that the fraction methanol extract had stronger anti-urease (12.52%) and anticholinesterase (69.02%) activity than other extracts.  The XTT results showed that fraction methanol extract had the most cytotoxic activity on MCF-7 cell lines (39.92%).  According to the results of in vivo anti-inflammatory activity, it was found that both fraction and direct methanol extracts exhibited close and significant anti-inflammatory activity. The fact that methanol extracts have significant biological activity suggests that these may be used as a natural source in the future.


Metrics Loading ...


Amanlou M, Dadkhah F, Salehnia A, Farsam H, Dehpour AR (2005). An anti-inflammatory and anti- nociceptive effects of hydroalcoholic extract of Satureja khuzistanica Jamzad extract. Journal of Pharmacy and Pharmaceutical Sciences 8(1):102-106.

Ayoub Z, Mehta A, Mishra SK, Ahirwal L (2017). Medicinal plants as natural antioxidants: A review. Journal of Botanical Society 48:1-9.

Benzie IF, Strain JJ (1996). The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Analytical Biochemistry 239(1):70-76.

Bezi N, SkoIbu M, Jaunk V (2005). Phytochemical composition and antimicrobial activity of Satureja montana L. and Satureja cuneifolia Ten. essential oils. Acta Botanica Croatica 64(2):313-322.

Butterfield DA, Halliwell B (2019). Oxidative stress, dysfunctional glucose metabolism and Alzheimer disease. Nature Reviews Neuroscience 20(3):148-160.

Ellman GL, Courtney KD, Andres Jr V, Featherstone RM (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology 7(2):88-90.

Fang YZ, Yang S, Wu G (2002). Free radicals, antioxidants and nutrition. Nutrition 18(10):872-879.

García-Rellána D, Verdeguer M, Salamone A, Amparo Blázquez M, Boira H (2016). Chemical composition, herbicidal and antifungal activity of Satureja cuneifolia essential oils from Spain. Natural Product Communications 11(6):841-844.

Hajhashemi V, Zolfaghari B, Yousefi A (2012). Antinociceptive and anti-inflammatory activities of Satureja hortensis seed essential oil, hydroalcoholic and polyphenolic extracts in animal models. Medical Principles and Practice 21(2):178-182.

Hanif M, Shoaib K, Saleem M, Rama NH, Zaib S, Iqbal J (2012). Synthesis, urease inhibition, antioxidant, antibacterial, and molecular docking studies of 1,3,4-oxadiazole derivatives. International Scholarly Research Network Pharmacology 2012:1-9.

Henstridge CM, Hyman BT, Spires-Jones TL (2019). Beyond the neuron–cellular interactions early in Alzheimer disease pathogenesis. Nature Reviews Neuroscience 20(2):94-108.

Holleczek B, Schöttker B, Brenner H (2020). Helicobacter pylori infection, chronic atrophic gastritis and risk of stomach and oesophagus cancer: results from the prospective population‐based esther cohort study. International Journal of Cancer 146:2773-2783.

Jafari F, Ghavidel F, Zarshenas MM (2016). A Critical overview on the pharmacological and clinical aspects of popular Satureja species. Journal of Acupuncture and Meridian Studies 9(3):118-127.

Kindl M, Blažeković, B, Bucar F, Vladimir-Knežević S (2015). Antioxidant and anticholinesterase potential of six Thymus Species. Evidence-Based Complementary and Alternative Medicine 2015:1-10.

Long JM, Holtzman DM (2019). Alzheimer disease: an update on pathobiology and treatment strategies. Cell 179(2):312-339.

Mimica-Dukić N, Simin N, Orčić D, Lesjak M, Knežević P, Aleksić V, Buzas K (2018). Therapeutic efficiency of essential oils against Helicobacter pylori infections. Facta Universitatis, Series Physics, Chemistry and Technology 16(1):29-32.

Mogana R, Adhikari A, Debnath S, Hazra S, Hazra B, Teng-Jin K, Wiart C (2014). The antiacetylcholinesterase and antileishmanial activities of Canarium patentinervium Miq., BioMed Research International 2014:1-7.

Oke F, Aslim B, Ozturk S, Altundag S (2009). Essential oil composition, antimicrobial and antioxidant activities of Satureja cuneifolia Ten. Food Chemistry 112(4):874-879.

Ozsoy N, Can A, Yanardağ R, Akev N (2008). Antioxidant activity of Smilax excels L. leaf extracts. Food Chemistry 110(3):571-583.

Ramos‐Tovar E, Muriel P (2020). Free Radicals, Antioxidants, nuclear factor‐e2‐related factor‐2 and liver damage. Journal of Applied Toxicology 40(1):151-168

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999). Antioxidant activity applying an improved abts radical cation decolorization assay. Free Radical Biology and Medicine 26(9-10):1231-1237.

Sadeghi I, Yousefzadi M, Behmanesh M, Sharifi M, Moradi A (2013). In vitro cytotoxic and antimicrobial activity of essential oil from Satureja intermedia. Iranian Red Crescent Medical Journal 15(1):70-74.

Taskin T, Çam ME, Taşkın D, Rayaman E (2019). In vitro and In vivo biological activities and phenolic characterization of Thymus praecox subsp. skorpilii var. skorpilii. Journal of Food Measurement and Characterization 13:536-544.

Taslimi P, Kokksal E, Goren AC, Bursal E, Aras A, Kılıc O, Alwasel S, Gulçin I (2020). Anti-Alzheimer, antidiabetic and antioxidant potential of Satureja cuneifolia and analysis of its phenolic contents by LC-MS/MS. Arabian Journal of Chemistry 13(3):4528-4537.

Taşkın D, Taşkın T, Rayaman E (2018). Phenolic composition and biological properties of Achillea nobilis L. subsp. neilreichii (Kerner) Formanek. Industrial Crops and Products 111:555-562

Tepe B, Cilkiz M (2015). A pharmacological and phytochemical overview on Satureja. Pharmaceutical Biology 54(3):375-412.

Vazquez E, Navarro M, Salazar Y, Crespo G, Bruges G, Osorio C, … Lopez M (2015). Systemic changes following carrageenan-induced paw inflammation in rats. Inflammation Research 64:333-342.

Wolf NB, Küchler S, Radowski MR, Blaschke T, Kramer KD, Weindi G (2009). Influences of opioids and nanoparticles on in vitro wound healing models. European Journal of Pharmaceutics and Biopharmaceutics 73(1):34-42.

Yousefzadi M, Riahi-Madvar A, Hadian J, Rezaee F, Rafiee R (2012). In vitro cytotoxic and antimicrobial activity of essential oil from Satureja sahendica. Toxicological and Environmental Chemistry 94(9):1735-1745.

How to Cite
TASKIN, T., DOGAN, M., CAM, M. E., SAHIN, T., & SENKARDES, I. (2020). In vitro anti-urease, antioxidant, anticholinesterase, cytotoxic and in vivo anti-inflammatory potential of Satureja cuneifolia Ten. Notulae Scientia Biologicae, 12(2), 222-232.
Research articles