Protective potentials of ethyl acetate-ethanolic fraction of Carica papaya leaves against acetaminophen-induced liver damage in rats


  • Hussein O. B. OLOYEDE University of Ilorin, Faculty of Life Sciences, Department of Biochemistry, Ilorin, Kwara State (NG)
  • Halimat Y. LUKMAN University of Ilorin, Faculty of Life Sciences, Department of Biochemistry, Ilorin, Kwara State (NG)
  • Musa O. SALAWU University of Ilorin, Faculty of Life Sciences, Department of Biochemistry, Ilorin, Kwara State (NG)



acetaminophen-induced liver damage; antioxidant; Carica papaya; ethyl acetate-ethanol fraction; flavonoids


The liver is an important organ performing vital roles that become affected when damaged. The protective potential of ethyl acetate-ethanolic fraction of Carica papaya leaves on acetaminophen-induced liver damage in female albino rats was investigated. Forty female rats were completely randomized into eight groups (A-H) of five rats each. Group A (control), received 1 ml of distilled water, group B (untreated) received 250 mgkg-1  bw of acetaminophen, group C received  silymarin (50 mgkg-1 bw) followed by actaminophen (250 mgkg-1 bw),  groups D-G received 100, 200, 400 and 600 mgkg-1  bw of ethyl acetate-ethanolic fraction of Carica papaya leaves respectively; followed by acetaminophen administration (250 mgkg-1 bw), while group H (toxicity control) received 600 mgkg-1 bw extract fraction daily. Ethyl acetate-ethanolic fraction of Carica papaya leaves significantly (p<0.05) decreased the activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma glutamyl transferase and bilirubin concentration and increased the concentrations of total protein and albumin in acetaminophen-induced liver damaged rats when compared with the untreated group. Prevention of liver damage was observed in the groups pre-administered 400 and 600 mgkg-1 bw of the extract fraction as well as the silymarin group. Activities of superoxide dismutase and catalase and the concentration of glutathione were significantly (p<0.05) increased while the concentration of malondialdehyde was significantly (p<0.05) decreased in rats pre-administered with silymarin or the extract fraction when compared with the acetaminophen-induced rats. Ethyl acetate-ethanolic fraction of Carica papaya leaves prevented acetaminophen-induced rat liver damage probably mainly due to the antioxidant constituents of the fraction.


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How to Cite

OLOYEDE, H. O. B. ., LUKMAN, H. Y., & SALAWU, M. O. (2020). Protective potentials of ethyl acetate-ethanolic fraction of Carica papaya leaves against acetaminophen-induced liver damage in rats. Notulae Scientia Biologicae, 12(3), 556–567.



Research articles
DOI: 10.15835/nsb12310629