Effects of irrigation management and seasonal stages on essential oil content and biomass of Origanum vulgare L.


  • Hugo THANER DOS SANTOS Federal University of Viçosa, Agricultural Engineering Department, Av. P.H. Rolfs, Campus UFV, Viçosa-MG (BR)
  • Renata ALCARDE SERMARINI University of São Paulo, Department of Math, Chemistry and Statistics, Av. Pádua Dias, 11, ESALQ-LCE, Piracicaba-SP (BR)
  • Maria A. MORENO-PIZANI Programa de Educação Continuada em Economia e Gestão de Empresas (PECEGE), Rua Alexandre Herculano, 120-T6, Piracicaba-SP (BR)
  • Patricia A. ALVES MARQUES University of São Paulo, Biosystems Engineering Department, Av. Pádua Dias, 11, ESALQ-LEB, Piracicaba-SP (BR)




dielectric properties, drip, greenhouse production, soil water content, spice


The responses of oregano plants to water limitation from soil and seasonal phenological cycle are not fully understood yet. The aim of the present research was to help understanding the production of oregano essential oil and biomass facing soil water deficit, which was studied in different seasons. Oregano was subjected to drip irrigation, the water deficit being assessed in the vegetative and pre-flowering stages, as well as whole cycle analysis, through water matric potentials in the soil. The matric potential -60.8 kPa adopted in the irrigation handling during the oregano cultivation interval, led to higher essential oil content and yield. The same potential applied during the oregano pre-flowering stage resulted in the best mean of oregano fresh biomass production. The best dry biomass production was reached by using the matric potential -91.2 kPa in the oregano pre-flowering irrigation management. Water restriction in the soil throughout the entire phenological cycle favored essential oil production, whereas the water restriction during the pre-flowering stage enabled high oregano dry biomass production. The highest values for biomass and essential oil productions were reached for the oregano plants cultivated during spring/summer.


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Abdelmajeed NA, Danial EN, Ayad HS (2013). The effect of environmental stress on qualitative and quantitative essential oil of aromatic and medicinal plants. Archives Des Sciences 66:100-120.

Asensio CM, Grosso NR, Juliani HR (2015). Quality characters, chemical composition and biological activities of oregano (Origanum spp.) essential oils from central and southern Argentina. Industrial Crops and Products 63:203-213.

Azizi A, Yan F, Honermeier B (2009) Herbage yield, essential oil content and composition of three oregano (Origanum vulgare L.) populations as affected by soil moisture regimes and nitrogen supply. Industrial Crops and Products 29:554-561.

Bafana A (2013). Diversity and metabolic potential of culturable root-associated bacteria from Origanum vulgare in sub-Himalayan region. World Journal of Microbiology and Biotechnology 29:63-74.

Baher ZF, Mirza M, Ghorbanli M, Rezaii MB (2002). The influence of waterstress on plant height, herbal and essential oil yield and composition in Satureja hortensis L. Flavour and Fragrance Journal 17:275-277.

Bahreininejad B, Razmjoo J, Mirza M (2013). Influence of water stress on morpho-physiological and phytochemical traits in Thymus daenensis. International Journal of Plant Production 7:151-156.

Basker D, Putievsky E (1978). Seasonal variation in the yields of herb and essential oil in some Labiatae species. Journal of Horticultural Science 53:179-183.

Batista SCO, Carvalho DF, Rocha HS, Thaner Dos Santos H, Medici LO (2013). Production of lettuce automatically watered with a low-cost controller. International Journal of Food, Agriculture and Environment 11:485-489.

Bekhradi F, Luna MC, Delshad M, Jordan MJ, Sotomayor JA, Martínez-Conesa C, Gil MI (2015). Effect of deficit irrigation on the postharvest quality of different genotypes of basil including purple and green Iranian cultivars and a Genovese variety. Postharvest Biology and Technology 100:127-135.

Box GEP, Hunter JS, Hunter WG (2005). Statistics for experimenters: design, innovation, and discovery. Wiley-Interscience, after Hoboken.

Breusch TS, Pagan AR (1979). A simple test for heteroscedasticity and random coefficient variation. Econometrica 47:1287-1294.

Burmeister J, Guttenberg HV (1960). Die ätherischen öle als produkt eines partiell anaeroben stoffwechsels. Planta Medica 8:1-33.

Busatta C, Mossi AJ, Rodrigues MRA, Cansian RL, Oliveira JV (2007). Evaluation of Origanum vulgare essential oil as antimicrobial agent in sausage. Brazilian Journal of Microbiology 38:610-616.

Chauhan NK, Singh S, Haider SZ, Lohani H (2013). Influence of phenological stages on yield and quality of oregano (Origanum vulgare L.) under the agroclimatic condition of doon valley (Uttarakhand). Indian Journal Pharmaceutical Science 75:89-493.

Chaves MM, Oliveira MM (2004). Mechanisms underlying plant resilience to water deficits: prospects for water-saving agriculture. Journal of Experimental Botany 55:2365-2384.

Chang X, Alderson PG, Wright CJ (2005). Effect of temperature integration on the growth and volatile oil content of basil (Ocimum basilicum L.). Journal of Horticultural Science & Biotechnology 80:593-598.

Circella G, Franz CH, Novak J, Resch H (1995). Influence of day length and leaf insertion on the composition of marjoram essential oil. Flavour and Fragrance Journal 10:371-374.

Clevenger JF (1928). Apparatus for the determination of volatile oil. Journal of the American Pharmaceutical Association 17:345-349.

Dardioti A, Karousou R, Lanaras T, Kokkni S (2012). Diversity of Satureja pilosa subsp. origanita essential oils: A new “oregano” from East Mediterranean. Biochemical Systematics and Ecology 40:178-183.

Davidenco V, Vega CRC, Piccardi MB, Arguello JA (2015). Development in Origanum ssp.: a simple phenological scale to determine thermal time requirements to critical reproductive events. Scientia Horticulturae 186:70-76.

Do TKT, Hadji-Minaglou F, Antoniotti S, Fernandez X (2015). Authenticity of essential oils. Trends in Analytical Chemistry 66: 146-157.

Dodd IC, Puértolas J, Huber K, Pérez-Pérez JG, Wright HR, Blackwell MS (2015). The importance of soil drying and re-wetting in crop phytohormonal and nutritional responses to deficit irrigation. Journal of Experimental Botany 66:2239-2252.

Du T, Kang S, Zhang J, Davies WJ (2015). Deficit irrigation and sustainable water- resource strategies in agriculture for China’s food security. Journal of Experimental Botany 66:2253-2269.

Economakis CD (1993). Effect of potassium on growth and yield of Origanum dictamnus L. in solution culture. Acta Horticulturae 331:339-344.

El Gendy NA, Leonardi M, Mugnaini L, Bertelloni F, Ebani VV, Nardoni S, ... Pistelli L (2015). Chemical composition and antimicrobial activity of essential oil of wild and cultivated Origanum syriacum plants grown in Sinai, Egypt. Industrial Crops and Products 67:201-207.

EMBRAPA (2013). Sistema brasileiro de classificação de solos. Brasília: Embrapa.

Ekren S, Sonmex C, Ozcakal E, Kurttas YSK, Bayram E, Gurgulu H (2012). The effect of different irrigation water levels on yield and quality characteristics of purple basil (Ocimum basilicum L.). Agricultural Water Management 109:155-161.

Farahani HA, Valadabadi SA, Daneshian J, Shiranirad AH, Khalvati MA (2009). Medicinal and aromatic plants farming under drought conditions. Journal of Horticulture and Forestry 1:86-92.

Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA (2009). Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development 29:185-212.

Figueiredo AC, Barroso JG, Pedro LG, Scheffer JJC (2008). Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour and Fragrance Journal 23:213-226.

Gomes DP, Carvalho DF, Almeida WS, Medici LO, Guerra JGM (2014). Organic carrot-lettuce intercropping using mulch and different irrigation levels. International Journal of Food, Agriculture and Environment 12:323-328.

Guidi L, Landi M (2014) Aromatic Plants: use and nutraceutical properties. In: Gurib-Fakim A (Ed). Novel plant bioresources: applications in food, medicine and cosmetics. Wiley, Bognor Regis.

Guvenalp Z, Turan M, Sumer Z (2010). Study on antitumoral activity of some chemical compounds isolated from Origanum vulgare ssp. Planta Medica 76:271.

Hassan FAS, Bazaid S, Ali EF (2013). Effect of deficit irrigation on growth, yield and volatile oil content on Rosmarinus officinalis L. plant. Journal of Medicinal Plants Studies 1:12-21.

Healy MJR (1956). The analysis of a factorial experiment with additional treatments. The Journal Agricultural Science 47:205-206.

Honermeier B, Ali S, Leschhorn B, Mahmood A, Ijaz M, Russo M, ... Zeller S (2013). Cultivation of medicinal and spice plants in Germany - a review. International Journal of Agriculture & Biology 15:1379-1388.

Khalil SES, El-Noemani ASA (2015). Effect of bio-fertilizers on growth, yield, water relations, photosynthetic pigments and carbohydrates contents of Origanum vulgare L. plants grown under water stress conditions. American-Eurasian Journal of Sustainable Agriculture 9:60-73.

Kofidis G, Bosabalidis AM, Moustakas M (2003). Contemporary seasonal and altitudinal variations of leaf structural features in oregano (Origanum vulgare L.). Annals of Botany 92:635-645.

Landi M, Guidi L (2015). Labiateae species in mediterranean environment: unrivaled sources of secondary metabolites. Italus Hortus 22:1-13.

Lemhadri A, Zeggwagh NA, Maghrani M (2004). Anti-hyperglycaemic activity of the aqueous extract of Origanum vulgare growing wild in Tafilalet region. Journal of Ethnopharmacology 92:251-256.

Létourneau G, Caron J, Anderson L, Cormier J (2015). Matric potential-based irrigation management of field-grown strawberry: effects on yield and water use efficiency. Agricultural Water Management 161:102-113.

Lukas B, Schmiderer C, Mitteregger U, Novak J (2010). Arbutin in marjoram and oregano. Food Chemistry 121:185-190.

Manukyan A (2011). Effect of growing factors on productivity and quality of lemon catmint, lemon balm and sage under soilless greenhouse production: I.

Drought stress. Medicinal and Aromatic Plant Science and Biotechnology 5:119-125.

Matraka M, Ninou E, Giannakoula A, Lazari D, Panou-Filotheou H, Bosabalidisa AM (2010). Effects of soil water content on Mentha spicata L. and Origanum dictamnus L. Israel Journal of Plant Sciences 58:229-239.

Meneses R, Ocazionez RE, Martinez JR, Stashenko EE (2009). Inhibitory effect of essential oils obtained from plants grown in Colombia on yellow fever virus replication in vitro. Annals of Clinical Microbiology and Antimicrobials 8:1-6.

Mijani S, Eskandri Nasrabadi S, Zarghani H, Ghias Abadi M (2013). Seed germination and early growth responses of hyssop, sweet basil and oregano to temperature levels. Notulae Scientia Biologicae 5:462-467.

Mortensen LM (2014). The effect of air temperature on growth of eight herb species. American Journal of Plant Sciences 5:1542-1546.

Neumann PM (1995). The role of cell wall adjustments in plant resistance to water deficits. Crop Science 35:1258-1266.

Osakabe Y, Osakabe K, Shinozaki K, Tran LSP (2014). Response of plants to water stress. Frontiers in Plant Science 5:1-8.

Paraskevakis N (2015). Effects of dietary dried Greek Oregano (Origanum vulgare ssp. hirtum) supplementation on blood and milk enzymatic antioxidant indices, on milk total antioxidant capacity and on productivity in goats. Animal Feed Science and Technology 209:90-97.

Penka M (1978). Influence of irrigation on the contents of effective substances in officinal plants. Acta Horticulturae 73:181-198.

R Development Core Team (2013). A Language and environment for statistical computing. Austria: R Foundation for Statistical Computing.

Raut S, Karuppayil SM (2014). A status review on the medicinal properties of essential oils. Industrial Crops and Products 62:250-264.

Rioba NB, Itulya FM, Said M, Dudai N, Bernstein N (2015). Effects of nitrogen, phosphorus and irrigation frequencyon essential oil content and composition of sage (Salvia officinalis L.). Journal of Applied Research on Medicinal and Aromatic Plants 2:21-29.

Said-Al Ahl H, Omer EA, Naguib NY (2009). Effect of water stress and nitrogen fertilizer on herb and essential oil of oregano. International Agrophysics 23:269-275.

Sangwan NS, FaroogI AHA, Shabid F, Sangwan RS (2001). Regulation of essential oil production in plants. Plant Growth Regulation 34:3-21.

Savini I, Arnone R, Catani MV, Avigliano L (2009). Origanum vulgare induces apoptosis in human colon cancer CaCo2 cells. Nutrition and Cancer 61:381-389.

Seghatoleslami M, Feizi H, Mousavi G, Berahmand A (2015). Effect of magnetic field and silver nanoparticles on yield and water use effi ciency of Carum copticum under water stress conditions. Polish Journal of Chemical Technology 17:110-114.

Shao HB, Chu LY, Jaleel CA, Zhao CX (2008). Water-deficit stress-induced anatomical changes in higher plants. Comptes Rendus Biologies 331:215-225.

Sivakumar D, Bautista-Bãnos S (2014). A review on the use of essential oils for postharvest decay control and maintenance of fruit quality during storage. Crop Protection 64:27-37.

Soil Survey Staff (1999). Soil taxonomy: a basic system of soil classification for making and interpreting soils surveys. Department of Agriculture. Soil Survey Division. Soil Conservation Service, Washington

Souza EL, Stamford TLM, Lima EO (2006). Sensitivity of spoiling and pathogen- food related bacteria to Origanum vulgare L. (Lamiaceae) essential oil. Brazilian Journal of Microbiology 37:527-532.

Telci I, Hisil Y (2008). Biomass yield and herb essential oil characters at different harvest stages of spring and autumn sown Coriandrum sativum. European Journal Horticultural Science 73:267-272.

Tibaldi G, Fontana E, Nicola S (2011). Growing conditions and postharvest management can affect the essential oil of Origanum vulgare L. ssp. hirtum (Link) Ietswaart. Industrial Crops and Products 34:1516-1522.

Trivellini A, Lucchesini M, Maggini R, Mosadegh H, Villamarin TS, Vernieri P, ... Pardossi A (2016). Lamiaceae phenols as multifaceted compounds: bioactivity, industrialprospects and role of “positive-stress”. Industrial Crops and Products 83:241-254.

Trivino MG, Johnson CB (2000). Season has a major effect on the essential oil yield response to nutrient supply in Origanum majorana. Journal of Horticultural Science & Biotechnology 75:520-527.

Torres LE, Brunetti PC, Baglio C, Bauza PG, Chaves AG, Massuh Y, ... Ojeda MS (2012). Field evaluation of twelve clones of oregano grown in the main production areas of Argentina: identification of quantitative trait with the highest discriminant value. International Scholarly Research Network Agronomy 2012:1-10.

Uhland RE (1951). Rapid method for determining soil moisture. Soil Science Society Proceedings 15:391-393.

Van Genuchten MTA (1980). A closed form equation for predicting the hydraulic conductivity of unsaturated soils. Soil Science Society of America Journal 44:892-898.

Voltolina G (2017). Piante Officinali Dalla coltivazione alla vendita nell’azienda agrícola multifunzionale Manuale agroerboristico. Verona: Edizioni L’Informatore Agrario.

Whalley WR, Ober ES, Jenkins M (2013). Measurement of the matric potential of soil water in the rhizosphere. Journal of Experimental Botany 64:3951-3963.

Xu DH, Li JH, Fang XW, Wang G (2007). Changes in soil water content in the rhizosphere of Artemisia ordosica: evidence for hydraulic lift. Journal of Arid Environments 69:545-553.




How to Cite

THANER DOS SANTOS, H., ALCARDE SERMARINI, R., MORENO-PIZANI, M. A., & ALVES MARQUES, P. A. (2020). Effects of irrigation management and seasonal stages on essential oil content and biomass of Origanum vulgare L. Notulae Scientia Biologicae, 12(1), 42–56. https://doi.org/10.15835/nsb12110588



Research articles
DOI: 10.15835/nsb12110588