Acute and Sub-Acute Toxicity of Cola millenii Leaf and Stem Bark Extracts in Albino Rats
In the present study, the toxicity of monkey kola (Cola millenii) leaf and stem bark extracts were investigated in laboratory animals. The rats were orally administered with the plant extracts at gradual doses of 10 mg/kg, 100 mg/kg, 1,000 mg/kg, 2,500 mg/kg, 3,500 mg/kg and 5,000 mg/kg b.w. and were closely monitored for 24 h. In sub-acute toxicity, the animals were given 1,000 mg/kg b.w. and 2,500 mg/kg b.w. for 28 days. Thereafter, the effect of the extracts on the weight and haematological parameters of the rats were determined. The rats did not show any symptoms of toxicity throughout the experiment. In the sub-acute toxicity assay, starting from week two, the groups administered with 1,000 mg/kg and 2,500 mg/kg stem bark extract had significant (p ≤ 0.05) lower weight compared with the other groups. In the haematological assay, there were no significant differences (p ≥ 0.05) between the RBC and platelet counts as well as PCV, haemoglobin, urea, cholesterol, creatinine, LDL, HDL and AST level of all the groups given the extract in comparison with the control. Albeit, the level of white blood cells count, was higher in the treated groups compared with the control with concomitant significant (p ≤ 0.05) increase in the ALT and ALP levels of the animals treated with the extracts compared with the control. Results show that Cola millenii leaf and stem bark extracts are safe for oral use, although, at higher doses, stem bark extract may cause liver damage.
Amupitan TA. (2013). Ethnobotanical survey of medicinal plants in Biu Local Government of Borno state, Nigeria. Comprehensive Journal of Herbs and Medicinal Plants 2(1): 7-11.
Barnett HA, O’Gara G. (2003). Diabetes and the Heart. Clinical Practice Series. Churchill. Livingstone. Edinburgh UK, pp. 11-32.
Gadanya AM, Sule MS, Atiku MK. (2011). Acute toxicity of ”agadagi‟ tea on rats. Bayero Journal of Pure and Applied Sciences 4(2): 147-149.
James S, James C (2002). Encyclopedia of Pharmaceutical Technology. Second edition, 2:70-79.
Kaufmann PB, Cseke LJ, Warber S, Duke JA, Brielmann HL. (1999). Natural Products From plants. CRC Press, Boca-Raton, Florida,pp. 183-205.
Larbie C, Arthur FKN, Woode E, Terlabi EO (2011). Evaluation of acute and sub-chronic toxicity of Annona Muricata (Linn.) aqueous extract in animals. European Journal of Experimental Biology, 1 (4):115-124
Lorke D (1983). A new approach to tropical acute toxicity testing. Arch. Toxicol, 53: 275-287
Mothana RAA, Abdo SAA, Hasson S, Athawab FMN, Alaghbari SAZ, Lindequist U (2010). Antimicrobial, antioxidant and cytotoxic activities and phytochemical screening of some Yemeni medicinal plants. Evidence Based Complementary and Alternative Medicine, 7(3): 232-330.
Nwankwo CE (2017). Antimicrobial and Spectroscopic Analyses of Cola millenii K. Schum. MSc Dissertation in the Department of Biological Sciences, College of Natural Sciences submitted to the School of Postgraduate Studies, Redeemer’s University, Ede, Nigeria.
Organization for Economic Cooperation and Development (OECD) (2008). Repeated dose oral toxicity test method in OECD Guidelines for testing of chemicals, No 407, Paris, France.
Patrick-Iwuanyanwu KC, Amadi U, Charles IA, Ayalogu EO (2012). Evaluation of acute and sub-chronic oral toxicity Study of baker cleansers bitters - A polyherbal drug on experimental rats. EXCLI Journal 11:632-640
Shetty A, Deepa S, Alwar MC (2007). Acute toxicity studies and determination of median lethal dose. J Med Aromatic Plant Sci, 93(7): 917.
World Health Organization (2012). Traditional medicine. Health topics. http://dx:doi.org/10:1155/2012/52487. Retrieved 20th August, 2018.
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