Analysis of secondary metabolites and in vitro evaluation of extracts of Carica papaya and Azadirachta indica leaves on selected human pathogens
Keywords:Candida albicans; Escherichia coli; Myricetin; Protodioscin; Staphylococcus aureus
Extraction of the leaves of Carica papaya (family Caricaceae) and Azadirachta indica (family Meliacea) were done using solvents with varying polarities (acetone, hexane and ethylacetate). The crude extracts were screened for phytoconstituents using the preliminary method and high-performance liquid chromatography (HPLC) for separation and quantification of the constituents. Susceptibility of three medically important microorganisms (Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Candida alblicans) to the solvent extracts was tested at 100 mg/mL, 50 mg/mL and 25 mg/mL concentrations the disc diffusion technique. Tannins, saponins, alkaloids, steroids, flavonoids and anthraquinone were present in all the solvent extracts of A. indica and C. papaya. Protein was present in all the solvent extracts of A. indica but absent in all the solvent extracts of C. papaya. Terpenoid was only present in hexane extract of A. indica but absent in other solvent extracts of A. indica and C. papaya. Flavonoid was present in all but only absent in ethylacetate extract of A. indica. Glycoside was present in all but absent in hexane extract of A. indica. Coumarin was only present in acetone extracts of both plants and absent in other solvent extracts of the two plants. Extracts of Carica papaya and Azadirachta indica displayed varying inhibitory activities (between 5.00-15.00 mm) against the organisms at all the tested concentrations. Acetone extract of A. indica produced zones of inhibition ranging from 5.00-14.00 mm while acetone extract of C. papaya produces a range of 4.00-10.00 mm. Also, hexane extract of A. indica produced inhibition range of 7.00-10.00 mm whereas hexane extract of C. papaya produces a range of 5.00-15.00 mm. However, ethylacetate extract of A. indica produced inhibition range of 5.00-13.67 mm, while ethylacetate extract of C. papaya produce a range of 5.00-15.00 mm. Different compounds quantified as different peaks by HPLC in the different solvent extracts of Carica papaya are acacic acid, genistein, protodioscin, betulinic acid, phorbolester, creptolepinone, brusatol and alpha ionone while the fractions from the solvent extracts of Azadirachta indica are myricetin, azadirachtol, azadirachtin a, pentadecane, phytol, azadirachnol, quercetin, b caryophyllen, alpha ionone, ascaridole, trams.b.farnes. Results obtained in this work indicated that all the solvent extracts of Carica papaya and Azadirachta indica contained active phytoconstituents and the extracts displayed good potentials at preventing diseases associated with the microorganisms tested in this work.
Alain DPB, Ernest K, Félix H, Yapi AAY, Joseph AD, Frédéric G (2010). Evaluation of the toxicity of a methanolic total extract of Mitragyna ciliataa natural antimalaric. International Journal of Biological and Chemical Science 4(3):770-781.
Amazu LU, Azikiwe CCA, Njoku CJ, Osuala FN, Nwosu PJC, Ajugwo AO, Enye JC (2010). Anti-inflammatory activity of the methanolic extract of the seeds of Carica papaya in experimental animals. Asian Pacific Journal of Tropical Medicine 3(11):884-886.
Anokwuru CP Anyasor GN, Ajibaye O, Fakoya O, Okebugwu P (2011). Effect of extraction solvents on phenolic, flavonoid and antioxidant activities of three Nigerian medicinal plants. Natural Science 9:53-61.
Arekemase MO, Oyeyiola GP, Balogun KI (2013). Assessment of Vernonia amygdalina on some selected pathogenic microorganisms from university of Ilorin teaching hospital. Journal of Microbiology, Biotechnology and Food Sciences 2(5):2360-2365.
Asen S (1984). High pressure liquid chromatography analysis of flavonoid chemical markers in petals from Gerbera flowers as an adjunct for cultivar and germplasm identification. Phytochemistry 23(11):2523-2526.
Baris O, Gulluce M, Sahin F, Ozer H, Kilic H, Ozkan H, ... Ozbek T (2006). Biological activities of the essential oil and methanol extract of Achillea Biebersteinii Afan. (Asteraceae). Turkish Journal of Biology 30:65-73.
Basri DF, Fan SH (2005). The potential of aqueous and acetone extracts of galls of Quercus infectoria as antibacterial agents. Indian Journal of Pharmacolology 37(1):26-29.
Bibi Y, Nisa S, Chaudhary F, Zia M (2011). Antibacterial activity of some selected medicinal plants of Pakistan. BMC Complementary Alternative Medicine 11:892-897.
Bisignino G, Sanogo R, Marino A, Aquino R, D’angelo V, Germano MP, ... Pizza C (1999). Antimicrobial activities of Mitracarpus scaber extract and isolated constituents. Letters in Applied Microbiology 30:105-108.
Boligon AA, Brum TF, Frohlich JK, Froeder ALF, Athayde ML (2012). HPLC/DAD profile and determination of total phenolics, flavonoids, tannins and alkaloids contents of Scutia buxifolia Reissek stem bark. Research Journal of Phytochemistry 6:84-91.
Dilika F, Afolayan AJ, Meyer JJM (1996). Comparative antibacterial activity of two Helichrysum species used in male circumcision in South Africa. South African Journal of Botany 63(3):158-159.
Eja ME, Arikpo GE, Enyi-Idoh KH, Ikpeme EM (2011). An evaluation of the antimicrobial synergy of garlic (Allium sativum) and Utazi (Gongronema latifolium) on E. coli and S. aureus. Malaysian Journal of Microbiology 7(1):45-49.
Ekam VS, Ebong PE, Umoh IB (2010). Phytochemical screening of activity directed extracts of Vernonia amygdalina leaves. Global Journal of Pure and Applied Sciences 16(1):151-154.
Eloff JN (1998). A sensitive and quick microplate method to determine the minimum inhibitory concentration of plant extracts for bacteria. Planta Medica 64:711-713.
Enyi-Idoh KH, Utasalo SJ, Epoke J, Arikpo GE, Eja ME (2012). Time-dependent evaluation of the antimicrobial and phytochemical properties of Vernonia amygdalina and Gongronema latifolium. The Internet Journal of Herbal and Plant Medicine 1(2):97-102.
Funmilayo VD, Olaniran OO, Kanife UC (2010). Antimicrobial activities of Aframomum melegueta (Alligator pepper). International Journal of Biology 2(2):126-131.
Gatsing D, Nkeugoauapi CFN, Nkah BFN, Kuiate JR, Tchouanguep FM (2010). Antibacterial activity, bioavailability and acute toxicity evaluation of the leaf extract of Alchornea cordifolia (Euphorbiaceae). International Journal of Pharmacology 6:173-182.
Goyal M, Nagori BP, Sasmal D (2008). Sedative and anticonvulsant effects of an alcoholic extract of Capparis decidua. Journal of Natural Medicines 63:375-379.
Gurunga S, Å kalko-Basnet N (2009). Wound healing properties of Carica papaya latex: In vivo evaluation in mice burn model. Journal of Ethnopharmacology 121:338-341.
Jawetz M, Adelberg S (2004). Medical microbiology. 3rd Edition. Churchill and Livingstone, Longman Group Limited, United Kingdom.
Koffi E, Sea T, Dodehe Y, Soro S (2010). Effect of solvent type on extraction of polyphenols from twenty-three Ivorian plants. Journal of Animal and Plant Sciences 5:550-558.
Mahesh B, Satish S (2008). Antimicrobial activity of some important medicinal plants against plant and human pathogens. World Journal of Agricultural Sciences 4:839-843.
Masoko P, Eloff JN (2006). Bioautography indicates the multiplicity of antifungal compounds from twenty-four Southern African Combretum species (Combretacea). African Journal of Biotechnolology 5(18):1625-1647.
Mathekga ADM, Meyer JJM, Horn MM, Drewes SE (2000). An acylated phloroglucinol with antimicrobial properties from Helichrysum caesptitium. Phytochemistry 53:93-96.
Michiels JA, Kevers C, Pincemail J, Defraigne JO, Dommes J (2012). Extraction conditions can greatly influence antioxidant capacity assays in plant food matrices. Food Chemistry 130(4):986-993.
Mohammad A, Shohreh S, Amirhossein A, Maryam G, Mohammad S (2010). Antidiabetic effects of aqueous fruits extract of Diospyros lotus L. on streptozotocin-induced diabetic rats and the possible morphologic changes in the liver, kidney and heart. Journal of Pharmacognosy 2(2):010-016.
Ncube N, Afolayan SA, Okoh AI (2008). Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. African Journal of Biotechnology 7:1797-1806.
Obadoni BO, Ochuko PO (2001). Phytochemical studies and comparative efficacy of the crude extract of some homeostatic plants in Edo and Delta states of Nigeria. Global Journal of Pure and Applied 8:203-208.
Ochei JO, Kolhatkar (2008). Medical laboratory science theory and practice. Tata McGrawHill Publishing Company, New York pp 603.
Ogundare AO, Adetuyi FC, Akinyosoye FA (2006). Antimicrobial activities of Vernonia tenoriana, African Journal of Biotechnology 5(18):1663-1668.
Okigbo RN, Ogbonnanya OU (2006). Antifungal effects of two tropical plants extract Ocimum gratissimum and Afromaomum melegueta on post-harvest yam Discorea spp. rot. African Journal of Biotechnology 5(9):727-731.
Perez C, Pauli M, Bazevque P (1990). An antibiotic assay by the agar well diffusion method. Acta Biologiae et Medicinae Experimentalis 15:113-115.
Philip K, Malek SNA, Wirakarnain S, Sim KS, Saravana K, Hong SL, ... Syarifah NSA (2009). Antimicrobial activity of some medicinal plants from Malaysia. American Journal of Applied Sciences 6(8):1613-1617.
Rojas JJ, Ochoa VJ, Ocampo SA, Monoz JF (2006). Screening for antimicrobial activity of ten medicinal plants used in Colombian folkloric medicine: a possible alternative in treatment of nonnosocomial infections. BMC Complementary Alternative Medicine 6:2.
Salie F, Eagles PFK, Lens HMJ (1996). Preliminary antimicrobial screening of four South African Asteraceae species. Journal of Ethnopharmacology 52(1):27-33.
Shariff ZU (2001). Modern herbal therapy for common ailments. Nature Pharmacy Series (Volume 1), Spectrum Books Limited, Ibadan, Nigeria in Association with Safari Books (Export) Limited, United Kingdom pp 9-84.
Springfield EP, Eagles PKF, Scott G (2005). Quality assessment of south African herbal medicines by means of HPLC fingerprinting. Journal of Ethnopharmacology 101:75-83.
Takazawa H, Tajima F, Miyashifa C (1982). An antifungal compound from shitake (Lentinus edodes) Yakugaku zasshi Journal of the Pharmaceutical Society of Japan 102(5):489-491.
Titanji VP, Zofou D, Ngemenya MN (2008). The antimalarial potential of medicinal plants used for the treatment of malaria in Cameroonian folk medicine. African Journal of Traditional Complementary and Alternative Medicines 5(3):302-321.
Trease GE, Evans WO (2007). Pharmacology. 11th Ed. Bailliere Tindall Ltd, London. (1989) pp 60-75.
Wong PYY, Kitts DD (2006). Studies on the dual antioxidant and antibacterial properties of parsley (Petroselinum crispum) and cilantro (Coriandrum sativum) extracts. Food Chemistry 97:505-515.
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