Evaluation of Leukocyte Mobilization and Platelet Aggregatory Effects of Ciprofloxacin, Lincomycin and Erythromycin in Wistar Albino Rats

Abstract

This study evaluated leucocyte mobilization and platelet aggregation effects of ciprofloxacin, lincomycin and Erythromycin in Wistar albino rats. Thirty-three female Wistar albino rats weighing 130-160 g and three male Wistar albino rats weighing 188-194 g, fed commercial growers’ mash and clean tap water were used. In leukocytes assay, thirty-three adult female Wistar rats were assigned into five treatments, eleven groups of three rats per group. First three groups were treated 10 mg kg^-1, 20 mg kg^-1 and 40 mg kg^-1 of ciprofloxacin, next three groups same doses of lincomycin, next three groups same doses of Erythromycin, the tenth group received Indomethacin 5 mg kg^-1 (reference drug), the last group 5 mg kg^-1 normal saline. Assay on platelet aggregator activity involved collection of blood samples (10 ml each, 1% EDTA, centrifuged at 3000 rpm for 10 minutes), test drugs dissolved in 1 mg/ml distilled water and   Indomethacin (the reference drug). The three antibiotics significantly reduced leucocyte mobilization into the affected tissue. They all had their maximum inhibitory effects at the highest dose (40 mg kg^-1) compared with indomethacin. Erythromycin 40 mg kg^-1 showed the highest inhibitory effect on leucocyte migration. Whereas ciprofloxacin and erythromycin had stepwise increase in absorbance from time 0 secs through to time 120 secs, lincomycin showed a sharp decrease in the absorbance at around 30 seconds followed by a continuous increase up to 120 seconds. The testing drugs prevented leukocyte mobilization also had stepwise increase in absorbance from time 0 secs through to time 120 secs in platelet aggregatory activity assay to some extent.