Evaluation of Leukocyte Mobilization and Platelet Aggregatory Effects of Ciprofloxacin, Lincomycin and Erythromycin in Wistar Albino Rats
This study evaluated leucocyte mobilization and platelet aggregation effects of ciprofloxacin, lincomycin and Erythromycin in Wistar albino rats. Thirty-three female Wistar albino rats weighing 130-160 g and three male Wistar albino rats weighing 188-194 g, fed commercial growers’ mash and clean tap water were used. In leukocytes assay, thirty-three adult female Wistar rats were assigned into five treatments, eleven groups of three rats per group. First three groups were treated 10 mg kg-1, 20 mg kg-1 and 40 mg kg-1 of ciprofloxacin, next three groups same doses of lincomycin, next three groups same doses of Erythromycin, the tenth group received Indomethacin 5 mg kg-1 (reference drug), the last group 5 mg kg-1 normal saline. Assay on platelet aggregator activity involved collection of blood samples (10 ml each, 1% EDTA, centrifuged at 3000 rpm for 10 minutes), test drugs dissolved in 1 mg/ml distilled water and Indomethacin (the reference drug). The three antibiotics significantly reduced leucocyte mobilization into the affected tissue. They all had their maximum inhibitory effects at the highest dose (40 mg kg-1) compared with indomethacin. Erythromycin 40 mg kg-1 showed the highest inhibitory effect on leucocyte migration. Whereas ciprofloxacin and erythromycin had stepwise increase in absorbance from time 0 secs through to time 120 secs, lincomycin showed a sharp decrease in the absorbance at around 30 seconds followed by a continuous increase up to 120 seconds. The testing drugs prevented leukocyte mobilization also had stepwise increase in absorbance from time 0 secs through to time 120 secs in platelet aggregatory activity assay to some extent.
Bhattacherjee P, Williams RN, Eakins KE (1983). A comparison of the ocular anti-inflammatory activity of steroidal and non-steroidal compounds in the rat. Investigative Ophthalmology and Visual Science 24:1143-1146.
Bollati M, Gaita F, Anselmino M (2011). Antiplatelet combinations for prevention of atherothrombotic events. Vascular Health Risk Management 7:23-30.
Born GVR, Cross MJ (1963). The aggregation of blood platelets. Journal of Physiology 168:178-195.
Camilleri E, Jacquin L, Paganelli F, Bonello L (2011). Personalized antiplatelet therapy: review of the latest clinical evidence. Current Cardiology Reports 13(4):296-302.
Dale DC, Boxer L, Liles CW (2008). The phagocytes: neutrophils and monocytes. Blood 112:935-945.
Hoff J (2000). Methods of blood collection in the mouse. Laboratory Animal 29(10):47-53.
Kei AA, Florentin M, Mikhailidis DP, Elisaf MS, Liberopoulos EN (2011). Review: antiplatelet drugs: what comes next? Clinical Applied Thrombosis Hemostasis 17(1):9-26.
Li Z, Delaney MK, O’Brien KA, Du X (2010). Signaling during platelet adhesion and activation. Arteriosclerosis, Thrombosis, and Vascular Biology 30(12):2341-2349.
Ribeiro RA, Flores CA, Cunha FQ, Ferreira SH (1991). IL-8 causes in vivo neutrophil migration by a cell dependent mechanism. Immunology 73:472-477.
Varga-Szabo D, Braun A, Nieswandt B (2009). Calcium signaling in platelets. Journal of Thrombosis and Haemostasis 7(7):1057-66.
Vega MA, Corbi AL (2006). Human macrophage activation: Too many functions and phenotypes for a single cell type. Immunologia 25:248-272.
Papers published in Notulae Scientia Biologicae are Open-Access, distributed under the terms and conditions of the Creative Commons Attribution License.
© Articles by the authors; licensee SMTCT, Cluj-Napoca, Romania. The journal allows the author(s) to hold the copyright/to retain publishing rights without restriction.
Open Access Journal - the journal offers free, immediate, and unrestricted access to peer-reviewed research and scholarly work, due SMTCT supports to increase the visibility, accessibility and reputation of the researchers, regardless of geography and their budgets. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.