Endophytic Bacteria: Hidden Protective Associates of Plants against Biotic and Abiotic Stresses
An endophyte is a microorganism, usually bacterium or fungus, which lives within the internal tissue of a host plant, causing no apparent harm. Some characteristics of an endophyte include ability to promote plant growth and to confer plant tolerance to biotic and abiotic stresses. Endophytic bacteria spread across many phyla including the Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria. Endophytic bacteria are recruited by hosts and they get attached to the surface and eventually find their way into the internal tissues where they spread to the intercellular spaces of host plants. These bacteria have been isolated and characterized from different plants. Currently, culture-independent methods such as sequencing of the 16S rRNA gene or metalogenomics are used for identification and characterization of endophytes. The mechanisms by which the endophyte-induced plant protection is brought about can be through direct and/or indirect methods. The direct strategy antagonizes phytopathogens by the production of chemical substances while the indirect mechanisms improve resistance of hosts to pathogens. Global gene expression studies on some common endophytic bacteria implicated these direct and indirect strategies of bacterial-induced protection. More research should be geared towards how the economic importance of endophytic bacteria could be utilized to enhance global food security.
Abadi VAJ, Sepehri M (2015). Effect of Piriformospora indica and Azotobacter chroococcum on mitigation of zinc deficiency stress in wheat (Triticum aestivum L.). Symbiosis 69(1):9-19.
Alquéres SM, Oliveira JH, Nogueira EM, Guedes HV, Oliveira PL, Câmara F, ... Martins OB (2010). Antioxidant pathways are up-regulated during biological nitrogen fixation to prevent ROS-induced nitrogenase inhibition in Gluconacetobacter diazotrophicus. Archives of Microbiology 192(10):835-841.
Azevedo JL, Maccheroni J, Jr. Pereira O, Ara WL (2000). Endophytic microorganisms: a review on insect control and recent advances on tropical plants. Electronic Journal of Biotechnology 3(1):15-16.
Aznar A, Chen NW, Thomine S, Dellagi A (2015). Immunity to plant pathogens and iron homeostasis. Plant Science 240:90-97.
Balsanelli E, Tuleski TR, de Baura VA, Yates MG, Chubatsu LS, de Oliveira PF, de Souza EM, Monteiro RA (2013). Maize root lectins mediate the Interaction with Herbaspirillum seropedicae via N-Acetyl glucosamine residues of lipopolysaccharides. PLoS ONE 8(10):e77001.
Bastian F, Cohen A, Piccoli P, Luna V, Baraldi R, Bottini R (1998). Production of indole-3-acetic acid and gibberellins A1 and A3 by Acetobacter diazotrophicus and Herbaspirillum seropedicae in chemically-deï¬ned culture media. Plant Growth Regulator 24(1):7-11.
Beltran-Garcia MJ, White JF Jr, Prado FM, Prieto KR, Yamaguchi LF, Torres MS, ... Di Mascio P (2014). Nitrogen acquisition in Agave tequilana from degradation of endophytic bacteria. Scientific Reports 4:6938.
Chaudhry V, Sharma S, Bansal K, Patil PB (2017). Glimpse into the genomes of rice endophytic bacteria: Diversity and distribution of firmicutes. Frontiers in Microbiology 7:2115.
Cho HS (2007). Interference of quorum sensing and virulence of the rice pathogen Burkholderia glumae by an engineered endophytic bacterium. FEMS Microbiology Ecology 60(1):14-23.
Cocq K, Gurr SJ, Hirsch PR, Mauchline TH (2017). Exploitation of endophytes for sustainable agricultural intensification. Molecular Plant Pathology 18(3):469-473.
Cole JR, Chai B, Marsh TL, Farris RJ, Wang Q, Kulam SA, ... Chandra S (2003). The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Research 31(1):442-443.
Compant S, Reiter B, Nowak J, Sessitsch A, Clément C, Barka EA (2005). Endophytic colonization of Vitis vinifera L. by plant growth-promoting bacterium Burkholderia sp. Strain PsJN. Applied Environmental Microbiology 71(4):1685-1693.
Conn V, Walker A, Franco CM (2008). Endophytic actinobacteria induce defense pathways in Arabidopsis thaliana. Molecular Plant Microbe Interaction 21(2):208-218.
Costa LEO, Queiroz MV, Borges AC, Moraes CA, Araújo EF (2012). Isolation and characterization of endophytic bacteria from the leaves of common bean (Phaseolus vulgaris). Brazilian Journal of Microbiology 43(4):1562-1575.
Cullimore DR (2000). Practical atlas for bacterial identification. Florida; CRC Press, Florida pp 209.
D’Alessandro M, Erb M, Ton J, Brandenburg A, Karlen D, Zopfi J, Turlings TCJ (2014). Volatiles produced by soil-borne endophytic bacteria increase plant pathogen resistance and affect tritrophic interactions. Plant, Cell and Environment 37(4):813-826.
Demain AL (2000). Microbial natural products: A past with a future. In: Wrigley SK, Hayes MA, Thomas R, Chrystal EJT, Nicholson N (Eds). Biodiversity: New Leads for Pharmaceutical and Agrochemical Industries. The Royal Society of Chemistry, Cambridge, United Kingdom pp 3-16.
Forchetti G, Masciarelli O, Alemano S, Alvarez D, Abdala G (2007). Endophytic bacteria in sunflower (Helianthus annuus L.): isolation, characterization, and production of jasmonates and abscisic acid in culture medium. Applied Microbiology and Biotechnology 76(5):1145-1152.
Gaiero JR, McCall CA, Thompson KA, Day NJ, Best AS, Dunï¬eld KE (2013). Inside the root microbiome: Bacterial root endophytes and plant growth promotion. American Journal of Botany 100(9):1738-1750.
Gao F, Dai C, Liu X (2010). Mechanisms of fungal endophytes in plant protection against pathogens. African Journal of Microbiology Research 4(13):1346-1351.
Ghadin N, Zin NM, Sabaratnam V, Badya N, Basri DF, Lian HH, Sidik NM(2008). Isolation and characterization of a novel endophytic Streptomyces SUK 06 with antimicrobial activity from Malaysian plant. Asian Journal of Plant Science 7(2):189-194.
Gherbawy Y, Hassan S (2012). Molecular characterization of endophytic bacteria from metal hyperaccumulator aquatic plant (Eichhornia crassipes) and its role in heavy metal removal. Geomicrobiology Journal 29(10):906-915.
Glassner H, Zchori-Fein E, Yaron S, Sessitsch A, Sauer U, Compant S (2017). Bacterial niches inside seeds of Cucumis melo L. Plant and Soil 422(1-2):101-113.
Gunatilaka AAL (2006). Natural products from plant-associated microorganisms: distribution, structural diversity, bioactivity and implications of their occurrence. Journal of Natural Product 69(3):509-526.
Hallmann J, Quadt-Hallmann A, Rodrıguez-Kabana R, Kloepper JW (1998). Interactions between Meloidogyne incognita and endophytic bacteria in cotton and cucumber. Soil Biology and Biochemistry 30(7):925-937.
Hardoim PR, van Overbeek LS, Berg G, Pirttilä AM, Compant S, Campisano A, ... Sessitsch A (2015). The hidden world within plants: Ecological and evolutionary considerations for deï¬ning functioning of microbial endophytes. Microbiology and Molecular Biology Reviews 79(3):293-320.
Heinig U, Scholz S, Jennewein S (2013). Getting to the bottom of taxol biosynthesis by fungi. Fungal Diversity 60(1):161-170.
Idnurm A, Meyer V (2014). Welcome to fungal biology and biotechnology. Fungal Biology and Biotechnology 1:8.
James EK, Gyaneshwar P, Mathan N, Barraquio WL, Reddy PM, Iannetta PP, ... Ladha JK (2002). Infection and colonization of rice seedlings by the plant growth-promoting bacterium Herbaspirillum seropedicae Z67. Molecular Plant Microbe Interaction 15(9):894-906.
Kandel SL, Joubert PM, Doty SL (2017). Bacterial endophyte colonization and distribution within plants. Microorganisms 5: 77.
Kawasaki A, Donn S, Ryan PR, Mathesius U, Devilla R, Jones A, Watt M (2016). Microbiome and exudates of the root and rhizosphere of Brachypodium distachyon, a model for wheat. PLoS One 11:e0164533.
Kiraly L, Barna B, Kiraly Z (2007). Plant resistance to pathogen infection: forms and mechanisms of innate and acquired resistance. Journal of Phytopathology 155(7-8):385-396.
Kloepper JW, Ryu CM (2006). Bacterial endophytes as elicitors of induced systemic resistance. In: Schulz BJE, Boyle CJC, Sieber TN (Eds). Microbial Root Endophytes. Springer-Verlag, Berlin pp 33-52.
Kwak M (2012). Complete genome sequence of the endophytic bacterium Burkholderia sp. strain KJ006. Journal of Bacteriology 194(16):4432-4433.
Lata R, Chowdhury S, Gond SK, White Jr. JF (2018). Induction of abiotic stress tolerance in plants by endophytic microbes. Letters in Applied Microbiology 66(4):268-276.
Lehtonen PT, Helander M, Siddiqui SA, Lehto K, Saikkonen K (2006). Endophytic fungus decreases plant virus infections in meadow ryegrass (Lolium pratense). Biology Letters 2(4):620-623.
Miliute I, Buzaite O, Baniulis D, Stanys V (2015). Bacterial endophytes in agricultural crops and their role in stress tolerance: a review. Zemdirbyste-Agriculture 102(4):465-478.
Parakhia MV, Tomar RS, Malaviya BJ, Dhingani RM, Rathod VM, ... Golakiya BA (2014). Draft genome sequence of the endophytic bacterium Enterobacter sp. MR1, isolated from drought tolerant plant (Buteamonosperma). Indian Journal of Microbiology 54(1):118-119.
Perez-Pantoja D, Donoso R, Agullo L, Cordova M, Seeger M, Pieper DH, Gonzalez B(2012). Genomic analysis of the potential for aromatic compounds biodegradation in Burkholderiales. Environmental Microbiology 14(5):1091-1117.
Petriacq P, Williams A, Cotton A, McFarlane AE, Rolfe SA, Ton J (2017). Metabolite proï¬ling of non-sterile rhizosphere soil. The Plant Journal 92(1):147-162.
Piromyou P, Songwattana P, Greetatorn, T, Okubo T, Kakizaki KC, Prakamhang J, ... Minamisawa K (2015). The type III secretion system (T3SS) is a determinant for rice-endophyte colonization by non-photosynthetic Bradyrhizobium. Microbes and Environment 30:291-300.
Romero FM, Marina M, Pieckenstain FL (2014). The communities of tomato (Solanum lycopersicum L.) leaf endophytic bacteria, analyzed by 16S-ribosomal RNA gene pyrosequencing. FEMS Microbiology Letters 351:187-219.
Ryan RP, Germaine K, Franks A, Ryan DJ, Dowling DN (2008). Bacterial endophytes: recent developments and applications. FEMS Microbiology Letters 278(1):1-9.
Rybakova D, Cernava T, Köberl M, Liebminger S, Etemadi M, Berg G (2016). Endophytes-assisted biocontrol: novel insights in ecology and the mode of action of Paenibacillus. Plant and Soil 405(1-2):125-140.
Santi C, Bogusz D, Franche C (2013). Biological nitrogen ï¬xation in non-legume plants. Annals of Botany 111(5):743-767.
Sessitsch A, Hardoim P, Döring J, Weilharter A, Krause A, Woyke T, ... Rahalkar M (2012). Functional characteristics of an endophyte community colonizing rice roots as revealed by metagenomic analysis. Molecular Plant Microbe Interactions 25(1):28-36.
Shittu HO, Castroverde DMC, Nazar RN, Robb J (2009). Plant-endophyte interplay protects tomato against a virulent Verticillium. Planta 229(2):415-426.
Shittu HO (2012). The concept of genomics: A review. Nigerian Journal of Botany 25(1):79-92.
Shittu HO, Aghogban ON, Igiehon E (2017). Plant pathogen attack strategies. Journal of Phytopathology and Plant Health 4(1):1-9.
Shittu HO, Obiazikwor OH (2018). Arms race between phytopathogens and host plants: A similitude of two nations at war. Journal of Microbiology, Biotechnology and Food Science 7(6):646-650.
Smit S, Widmann J, Knight R (2007). Evolutionary rates vary among rRNA structural elements. Nucleic Acids Research 35(10):3339-3354.
Strobel G, Daisy B, Castillo U, Harper J (2004). Natural products from endophytic microorganisms. Journal of Natural Product 67(2):257-268.
Taghavi S, Garafola C, Monchy S, Newman L, Hoffman A, Weyens N, ... van der Lelie D (2009). Genome survey and characterization of endophytic bacteria exhibiting a beneficial effect on growth and development of poplar trees. Applied and Environmental Microbiology 75(3):748-757.
Taghavi S, Van Der Lelie D, Hoffman A, Zhang YB, Walla MD, Vangronsveld J, ... Monchy S (2010). Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638. PLoS Genetics 6:e1000943.
Tariq M, Zahid M, Hameed S, Zafar M, Yasmeen T (2014). Molecular characterization and identiï¬cation of plant growth promoting endophytic bacteria isolated from the root nodules of pea (Pisum sativum L.). World Journal of Microbiology and Biotechnology 30(2):719-725.
Tamosiune I, Baniulis D, Stanys V (2017). Role of endophytic bacteria in stress tolerance of agricultural plants: Diversity of microorganisms and molecular mechanisms. In: Kumar V, Kumar M, Sharma S, Prasad R (Eds). Probiotics in Agroecosystem. Springer, Singapore pp 26-42.
Tian P, Nan Z, Li C (2008). Effect of the endophyte Neotyphodium lolii on susceptibility and host physiological response of perennial ryegrass to fungal pathogens. European Journal of Plant Pathology 122(4):593-602.
Tjamos SE, Flemetakis E, Paplomatas EJ, Katinakis P (2005). Induction of resistance to Verticillium dahliae in Arabidopsis thaliana by the biocontrol agent K-165 and pathogenesis-related proteins gene expression. Molecular Plant-Microbe Interaction 18(6):555-561.
Tran PN, Tan NE, Lee YP, Gan HM, Polter SJ, Dailey LK, ... Savka MA (2015). Whole-genome sequence and classiï¬cation of 11 endophytic bacteria from poison ivy (Toxicodendron radicans). Genome Announcements 3(6):e01319-15.
Truyens S, Weyens N, Cuypers A, Vangronsveld J (2014). Bacterial seed endophytes: genera, vertical transmission and interaction with plants. Environmental Microbiology Reports 7(1):40-50.
Turner TR, James EK, Poole PS (2013). The plant microbiome. Genome Biology 14:209-213.
Van Loon LC (2006). Significance of inducible defense-related proteins in infected plants. Annual Review of Phytopathology 44:135-162.
Wemheuer F, Kaiser K, Karlovsky P, Daniel R, Vidal S, Wemheuer B (2017). Bacterial endophyte communities of three agricultural important grass species differ in their response towards management regimes. Scientific Reports 7:409-414.
Xu XH, Su ZZ, Wang C, Kubicek CP, Feng XX, Mao LJ, ... Zhang CL (2014). The rice endophyte Harpophora oryzae genome reveals evolution from a pathogen to a mutualistic endophyte. Scientific Reports 4:5783.
Xu Z, Jiang Y, Jia B, Zhou G (2016). Elevated-CO2 response of stomata and its dependence on environmental factors. Frontiers in Plant Science 7:657.
Zachow C, Jahanshah G, de Bruijn I, Song C, Ianni F, Pataj Z, ... Gross H (2015). The novel lipopeptide poaeamide of the endophyte Pseudomonas poae RE_1-1-14 is involved in pathogen suppression and root colonization. Molecular Plant Microbe Interactions 28(7):800-810.
Zuniga A, Poupin MJ, Donoso R, Ledger T, Guiliani N, Gutiérrez RA, Gonzalez B (2013). Quorum sensing and indole-3-acetic acid degradation play a role in colonization and plant growth promotion of Arabidopsis thaliana by Burkholderia phytoï¬rmans PsJN. Molecular Plant-Microbe Interactions 26(5):546-553.
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