Impact of Hydrogen Peroxide on the Secondary Metabolites, Enzyme Activities and Photosynthetic Pigment Accumulation of Vigna unguiculata L. (Walp)
The effects of exogenous application of hydrogen peroxide on the secondary metabolites, enzyme activities and photosynthetic pigment accumulation of Vigna unguiculata was investigated. The hereby study was carried out under a screen house to minimize extraneous factors. The experiment was laid down in completely randomized design (CRD) with eight replicates. After two weeks of sowing when the seedlings become fully established, the sample pots were divided into two regimes. The first regimes were supplied daily with 500 mL of 0.1 mM of hydrogen peroxide and the second regime was watered daily with tap water. After fifth week of sowing, the leaves of Vigna unguiculata from each regime were randomly selected and harvested. Secondary metabolites, enzyme assay and photosynthetic pigment screening was carried out on the harvested leaves. The impact of hydrogen peroxide on the parameters studied was done using one way analysis of variance. Mean values were separated using Fisher’s LSD at p≤0.05. The results showed that hydrogen peroxide strongly increased the accumulation of secondary metabolites such as flavonoids, glycosides, saponins, carbohydrates and alkaloids. It also induced α-amylase and lipase activities than the control plants and promotes the accumulation and biosynthesis of chlorophylls and carotenoids. In conclusion, hydrogen peroxide could be used as potential molecule to improve some secondary metabolites (flavonoids, glycosides, saponins, carbohydrates and alkaloids), α-amylase and lipase activities and photosynthetic pigments.
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