Antimicrobial Activity of Olea europaea Fatty Oil against Multi-Drug Resistant and Biofilm Forming Microorganisms

  • Haifaa Bawie NAJEE University of Bucharest, Faculty of Biology, Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB (RO)
  • Dunya ALKURJIA University of Bucharest, Faculty of Biology, Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB (RO)
  • Othman ALMAHDAWY University of Bucharest, Faculty of Biology, Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB (RO)
  • Crina KAMERZAN Research Institute of the University of Bucharest-ICUB (RO)
  • Luminita MARUTESCU University of Bucharest, Faculty of Biology, Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB (RO)
  • Irina GHEORGHE University of Bucharest, Faculty of Biology, Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB (RO)
  • Marcela POPA University of Bucharest, Faculty of Biology, Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB (RO)
  • Mariana Carmen CHIFIRIUC University of Bucharest, Faculty of Biology, Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB (RO)
  • Veronica LAZĂR University of Bucharest, Faculty of Biology, Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUB (RO)
Keywords: antimicrobial activity; anti-biofilm activity; efflux pump; fatty oil; flow cytometry

Abstract

The aim of the present study was to investigate the potential antimicrobial activity of Olea europaea fatty oil against a collection of bacterial (Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa) and fungal (Candida albicans) clinical isolates. The antimicrobial and antibiofilm activity were evaluated by broth microdilution method for establishing the minimal inhibitory concentration (MIC) and microtiter assay for determining the minimal biofilm eradication concentration (MBEC). Some of the potential microbial targets of the fatty oil were investigated by flow cytometry (FCM). The results obtained hereby revealed that Olea europaea fatty oil inhibited microbial planktonic growth (MICs values of 5.23-41.8 mg/ mL) and biofilm development on inert substrata (MBEC values of 1.31-20.9 mg/ mL). The FCM measurements confirmed that the analyzed oil induced microbial membrane damages and inhibited microbial efflux pump activity.

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Published
2018-12-21
How to Cite
NAJEE, H. B., ALKURJIA, D., ALMAHDAWY, O., KAMERZAN, C., MARUTESCU, L., GHEORGHE, I., POPA, M., CHIFIRIUC, M. C., & LAZĂR, V. (2018). Antimicrobial Activity of Olea europaea Fatty Oil against Multi-Drug Resistant and Biofilm Forming Microorganisms. Notulae Scientia Biologicae, 10(4), 498-502. https://doi.org/10.15835/nsb10410404
Section
Research articles
CITATION
DOI: 10.15835/nsb10410404