Evaluation of In Vitro Shoot Elongation and Rooting of Date Palm, and Determination of Physiological Characteristics of Regenerated Plantlets

Reda MEZIANI; Mouaad Amine MAZRI; Mahassine ARHAZZAL; Ilham BELKOURA; Chakib ALEM; Fatima JAITI

doi:10.15835/nsb11110402

Authors

Reda MEZIANI Institut National de la Recherche Agronomique, CRRA-Errachidia, UR Systèmes Oasiens, Laboratoire National de Culture des Tissus du Palmier Dattier, BP 2, Errachidia (MA)
Mouaad Amine MAZRI Institut National de la Recherche Agronomique, CRRA-Marrakech, UR Agro-Biotechnologie, Laboratoire de Biotechnologie Végétale, BP 533, Marrakech (MA)
Mahassine ARHAZZAL Ecole Nationale d’Agriculture, Département des Sciences de Base, Laboratoire de Culture In Vitro, BP S/40, Meknes (MA)
Ilham BELKOURA Ecole Nationale d’Agriculture, Département des Sciences de Base, Laboratoire de Culture In Vitro, Meknes (MA)
Chakib ALEM Université Moulay Ismail, Faculté des Sciences et Techniques, Errachidia (MA)
Fatima JAITI Université Moulay Ismail, Faculté des Sciences et Techniques, Errachidia (MA)

DOI:

https://doi.org/10.15835/nsb11110402

Keywords:

chlorophyll content, chlorophyll fluorescence, foliar surface, morphological characteristics, organogenesis, Phoenix dactylifera L., stomatal conductance

Abstract

The effects of various culture conditions on shoot elongation, rooting and plantlet acclimatization were tested. Adventitious shoots obtained through direct organogenesis of date palm (Phoenix dactylifera L.) cv. ‘Mejhoul’ were used as explants. The effects of culture medium texture, plant growth regulators, polyvinylpyrrolidone, adenine, myo-inositol, L-glutamine, and carbon source on in vitro plantlet quality and subsequent acclimatization were evaluated. The most effective culture medium was the semi-solid and half-strength Murashige and Skoog medium without plant growth regulators, supplemented with 30 g L^-1 sucrose. After 3 months of culture on this medium, the average shoot length was 13.6 cm, the average number of adventitious roots per shoot was 3.6, and the average root length was 3.85 cm. The survival rate of these plantlets in acclimatization was 90%. On the other hand, liquid medium, plant growth regulators, polyvinylpyrrolidone, adenine, myo-inositol and L-glutamine did not increase the survival rate during acclimatization. Along with these experiments, some physiological characteristics of the plantlets obtained in vitro were also determined. Chlorophyll content and fluorescence, foliar surface and stomatal conductance were measured after 3 months of culture in each medium. The ranges were as follows: Chlorophyll content, 11.7-31.8 CCI; chlorophyll fluorescence, 0.633-0.795; foliar surface, 7.35-13.29 cm²; and stomatal conductance, 10.3-36.0 mmol m^-2 s^-1. Interestingly, positive correlations between the physiological characteristics of the plantlets and their survival percentage in the glasshouse were revealed. The findings of this investigation will be valuable for large-scale and cost-saving production of date palm cv. ‘Mejhoul’ plants.

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References

Bhagyalakshmi, Singh NS (1995). Role of liquid versus agar-gelled media in mass propagation and ex vitro survival in bananas. Plant Cell Tissue and Organ Culture 41(1):71-73.

Burrows FJ, Milthorpe FL (1976). Stomatal conductance in the control of gas exchange. In: Kozlowski TT (Ed) Water deficits and plant growth, vol. IV. Academic Press, London New York, pp 103-152.

Cabrera HM, ArgandoÃ±a VH, ZúÃ±iga GE, Corcuera LJ (1995). Effect of infestation by aphids on the water status of barley and insect development. Phytochemistry 40(4):1083-1088.

Camen D, Beinșan C, Șumălan R, Pasc M (2010). Research on the effect of arbuscular mycorrhizae on some physiological indices of lettuce - Lactuca sativa L. Journal of Horticulture, Forestry and Biotechnology 14(3):128-130.

Derridj S, Wu BR, Stammitti L, Garrec JP, Derrien A (1996). Chemicals on the leaf surface, information about the plant available to insects. Entomologia Experimentalis et Applicata 80(1):197-201.

Filella I, PeÃ±uelas J, Seco R (2009). Short-chained oxygenated VOC emissions in Pinus halepensis in response to changes in water availability. Acta Physiologiae Plantarum 31(2):311-318.

Fki L, Bouaziz N, Kriaa W, Benjemaa-Masmoudi R, Gargouri-Bouzid R, Rival A, Drira N (2011). Multiple bud cultures of ‘Barhee’ date palm (Phoenix dactylifera) and physiological status of regenerated plants. Journal of Plant Physiology 168(14):1694-1700.

Jain SM (2012). Date palm biotechnology: Current status and prospective-an overview. Emirates Journal of Food and Agriculture 24(5):386-399.

Johnson DV (2011). Introduction: Date palm biotechnology from theory to practice. In: Jain SM, Al-Khayri JM, Johnson DV (Eds). Date palm biotechnology. Springer, Dordrecht pp 1-11.

Johnson HB, Rowlands PG, Ting IP (1979). Tritium and carbon-14 double isotope porometer for simultaneous measurements of transpiration and photosynthesis. Photosynthetica 13(4):409-418.

Jones HG (1992). Plants and microclimate: a quantitative approach to environmental plant physiology. Cambridge University Press.

Khan S, Bi Bi T (2012). Direct shoot regeneration system for date palm (Phoenix dactylifera L.) cv. ‘Dhakki’ as a means of micropropagation. Pakistan Journal of Botany 44(6):1965-1971.

Khierallah HSM, Bader SM (2007). Micropropagation of date palm (Phoenix dactylifera L.) var. ‘Mektoom’ through direct organogenesis. Acta Horticulturae 736:213-224.

Kiviharju E, Moisander S, Laurila J (2005). Improved green plant regeneration rates from oat anther culture and the agronomic performance of some DH lines. Plant Cell, Tissue and Organ Culture 81(1):1-9.

Lee B, Kim D, Ryu CM (2008). A super-absorbent polymer combination promotes bacterial aggressiveness uncoupled from the epiphytic population. The Plant Pathology Journal 24(3):283-288.

Lu MC (2005). Micropropagation of Vitis thunbergii Sieb. et Zucc., a medicinal herb, through high-frequency shoot tip culture. Scientia Horticulturae 107(1):64-69.

Machakova I, Zazimalova E, George EF (2008). Plant growth regulators I: Introduction; auxins, their analogues and inhibitors. In: George EF, Hall MA, De Klerk GJ (Eds). Plant propagation by tissue culture, 3rd edn. Springer, Dordrecht pp 175-204.

Mazri MA (2012). Effect of liquid media and in vitro pre-acclimatization stage on shoot elongation and acclimatization of date palm (Phoenix dactylifera L.) cv. ‘Najda’. Journal of Ornamental and Horticultural Plants 2(4):225-231.

Mazri MA (2013). Effect of basal medium, explants size and density on the in vitro proliferation and growth of date palm (Phoenix dactylifera L.) cultivar ‘16-bis’. Notulae Scientia Biologicae 5(3):332-337.

Mazri MA (2014). Effects of plant growth regulators and carbon source on shoot proliferation and regeneration in date palm (Phoenix dactylifera L.) ‘16-bis’. Journal of Horticultural Science and Biotechnology 89(4):415-422.

Mazri MA (2015). Role of cytokinins and physical state of the culture medium to improve in vitro shoot multiplication, rooting and acclimatization of date palm (Phoenix dactylifera L.) cv. ‘Boufeggous’. Journal of Plant Biochemistry and Biotechnology 24(3):268-275.

Mazri MA, Meziani R (2013). An improved method for micropropagation and regeneration of date palm (Phoenix dactylifera L.). Journal of Plant Biochemistry and Biotechnology 22(2):176-184.

Mazri MA, Meziani R (2015). Micropropagation of date palm: a review. Cell & Developmental Biology 4(3):160.

Mazri MA, Meziani R, El Fadile J, Ezzinbi A (2016). Optimization of medium composition for in vitro shoot proliferation and growth of date palm cv. ‘Mejhoul’. 3 Biotech 6(1):111.

Mazri MA, Belkoura I, Meziani R, Mokhless B, Nour S (2017). Somatic embryogenesis from bud and leaf explants of date palm (Phoenix dactylifera L.) cv. ‘Najda’. 3 Biotech 7(1):58.

Meziani R, Jaiti F, Mazri MA, Anjarne M, Ait Chitt M, El Fadile J, Alem C (2015). Effects of plant growth regulators and light intensity on the micropropagation of date palm (Phoenix dactylifera L.) cv. ‘Mejhoul’. Journal of Crop Science and Biotechnology 18(5):325-331.

Meziani R, Jaiti F, Mazri MA, Hassani A, Ben Salem S, ... Alem C (2016). Organogenesis of Phoenix dactylifera L. cv. ‘Mejhoul’: Influences of natural and synthetic compounds on tissue browning, and analysis of protein concentrations and peroxidase activity in explants. Scientia Horticulturae 204:145-152.

Mohamed MAH, Alsadon AA (2010). Influence of ventilation and sucrose on growth and leaf anatomy of micropropagated potato plantlets. Scientia Horticulturae 123(3):295-300.

Moshkov IE, Novikova GV, Hall MA, George EF (2008). Plant growth regulators III: gibberellins, ethylene, abscisic acid, their analogues and inhibitors; miscellaneous compounds. In: George EF, Hall MA, De Klerk GJ (Eds). Plant propagation by tissue culture, 3rd edn. Springer, Dordrecht pp 227-281.

Murashige T, Skoog FA (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Phys Planta Physiologia Plantarum 15(3):473-479.

Neto VBP, Otoni WC (2003). Carbon sources and their osmotic potential in plant tissue culture: does it matter? Scientia Horticulturae 97(3-4):193-202.

Newsholme P, Lima MMR, Procopio J, Pithon-Curi TC, ... Curi R (2003). Glutamine and glutamate as vital metabolites. Brazilian Journal of Medical and Biological Research 36(2):153-163.

Oliveira RS, Ma Y, Rocha I, Carvalho MF, Vosátka M, Freitas H (2016). Arbuscular mycorrhizal fungi are an alternative to the application of chemical fertilizer in the production of the medicinal and aromatic plant Coriandrum sativum L. Journal of Toxicology and Environmental Health A 79(7):320-328.

Rezali NI, Sidik NJ, Saleh A, Osman NI, Adam NAM (2017). The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme. Asian Pacific Journal of Tropical Biomedicine 7(2):151-156.

Sedra MH (2005). Phenological descriptors and molecular markers for the determination of true-to-type of tissue culture-derived plants using organogenesis of some Moroccan date palm (Phoenix dactylifera L.) varieties. Al Awamia 113:85-101.

Sedra MH (2011). Development of new Moroccan selected date palm varieties resistant to Bayoud and of good fruit quality. In: Jain SM, Al-Khayri JM, Johnson DV (Eds). Date palm biotechnology. Springer, Dordrecht pp 513-531.

Sedra MH (2015). Date palm status and perspective in Morocco. In: Al-Khayri JM, Jain SM, Johnson DV (Eds). Date Palm Genetic Resources and Utilization. Springer, Dordrecht pp 257-323.

Shabani F, Kumar L, Taylor S (2014). Projecting date palm distribution in Iran under climate change using topography, physicochemical soil properties, soil taxonomy, land use and climate data. Theoretical and Applied Climatology 118(3):553-567.

Skidmore DJ, Simons AJ, Bedi S (1988). In vitro culture of shoots of Pinus caribaea on a liquid medium. Plant Cell Tissue and Organ Culture 14(2):129-136.

Suthar RK, Habibi N, Purohit SD (2011). Influence of agar concentration and liquid medium on in vitro propagation of Boswellia serrata Roxb. Indian Journal of Biotechnology 10: 224-227.

Thorpe T, Stasolla C, Yeung EC, de Klerk GJ, Roberts A, George EF (2008). The components of plant tissue culture media II: organic additions, osmotic and pH effects, and support systems. In: George EF, Hall MA, De Klerk GJ (Eds). Plant propagation by tissue culture, 3rd edn. Springer, Dordrecht pp 115-173.

Van Staden J, Zazimalova E, George EF (2008). Plant growth regulators II: cytokinins, their analogues and antagonists. In: George EF, Hall MA, De Klerk GJ (Eds). Plant propagation by tissue culture, 3rd edn. Springer, Dordrecht pp 205-226.

Vernon LP, Seely GR (1966). The chlorophylls. Academic Press, New York.

Yaseen M, Ahmad T, Sablok G, Standardi A, Hafiz IA (2013). Review: role of carbon sources for in vitro plant growth and development. Molecular Biology Reports 40:2834-2849.

Zaid A, El-Korchi B, Visser HJ (2011). Commercial date palm tissue culture procedures and facility establishment. In: Jain SM, Al-Khayri JM, Johnson DV (Eds). Date palm biotechnology. Springer, Dordrecht pp 137-180.

Zouine J, El Hadrami I (2007). Effect of 2,4-D, glutamine and BAP on embryogenic suspension culture of date palm (Phoenix dactylifera L.). Scientia Horticulturae 112(2):221-226.

Zouine J, El Bellaj M, Meddich A, Verdeil J, El Hadrami I (2005). Proliferation and germination of somatic embryos from embryogenic suspension cultures in Phoenix dactylifera L. Plant Cell Tissue and Organ Culture 82(1):83-92.