Assessment of Genetic Diversity of Promising Castor Bean (Ricinus communis L.) Genotypes in Nigeria


  • Bolaji Zuluqurineen SALIHU National Cereals Research Institute, Research Operation Department, Badeggi, P.M.B. 8, Bida (NG)
  • Olamide Ahmed FALUSI Federal University of Technology, Department of Biological Sciences, P.M.B 65, Minna (NG)
  • Adeyinka Olufemi ADEPOJU University of Sierra Leone, Freetown, Fourah Bay College, Department of Biological Sciences (SL)
  • Ibrahim Wasiu AROLU Kaduna State University, Faculty of Agriculture, Crop Science Department, P.M.B. 2339, Kaduna (NG)
  • Oladipupo Yusuf DAUDU Federal University of Technology, Department of Biological Sciences, P.M.B 65, Minna (NG)
  • Dorcas Ropo ABEJIDE Federal University of Technology, Department of Biological Sciences, P.M.B 65, Minna (NG)
  • Christiana Oreoluwa OKE Kwara State University, Department of Plant and Environmental Biology, Malete, P.M.B.1530, Ilorin (NG)



agglomerative; castor oil plant; molecular markers; morphological descriptors


Castor oil plant (Ricinus communis L.) is an important oil crop with little research attention in Nigeria. In the present research, extent of genetic diversity among 20 Nigerian castor genotypes was determined using morphological descriptors and molecular markers. The genotypes were laid out on a randomized complete block design with three replicated plots. Molecular genotyping of the genotypes was carried out using genomic Simple Sequence Repeats (SSR). The genotypes revealed high divergence in seed colour, seed shape, seed mottle, seed caruncle and seed sizes. Seedling establishment varied from 70.18% (in Acc. 006) to 93.25% (Acc. 001) with average mean of 81.53%. Raceme length ranged from 15.90 cm to 29.54 cm with population mean of 20.80 cm. The highest seed yield (1222.98 kg/ha) was recorded in Acc. 001 and the least (611.46 kg/ha) was observed in Acc. 006. Seed oil content varied between 32.15% in Acc. 042 and 54.03% in Acc. 006. Agglomerative cluster dendrogram constructed from morphological data showed random distribution of the genotypes into three cluster groups irrespective of the sources/collection points. The genetic diversity based on SSR Marker Analysis revealed high average expected heterozygosity (0.74), Polymorphic information content (0.68), Nei’s gene diversity index (0.72) and Shannon's Information index (1.43). The dendrogram constructed from molecular data grouped the twenty genotypes into three groups at coefficient of 0.34. From these findings, it showed that the twenty genotypes evaluated are divergent in nature and they could serve as good genetic material for castor breeding in Nigeria.


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Ahmad F, Hanafi MM, Hakim MA, Rafii MY, Arolu IW, Akmar ASN (2015). Genetic divergence and heritability of 42 coloured upland rice genotypes (Oryza sativa) as revealed by microsatellites marker and agro-morphological traits. PLoS One 10(9):e0138246.

Allan G, Williams A, Rabinowicz PD, Chan AP, Ravel J, Keim P (2008). Worldwide genotyping of castor bean germplasm (Ricinus communis L.) using AFLPs and SSRs. Genetic Resources and Crop Evolution 55(3):365-378.

Amosun A, Salihu BZ, Shaahu A, Agboire SA, Apuyor OB, Oliseh A (2013). Castor germplasm collection and characterization. In: NCRI. Annual Research Review. Information and Documentation Unit NCRI Badeggi Nigeria, pp 121-153.

Anjani K (2012). Castor genetic resources: A primary gene pool for exploitation. Indian Crops Production 35(1):1-14.

Costa MND, Pereira WE, Bruno RDLA, Freire EC, Nóbrega MBDM, Milani M, Oliveira APD (2006). Genetic divergence on castor bean accesses and cultivars through multivariate analysis. Pesquisa Agropecuária Brasileira 41(11):1617-1622.

Dellaporta SL, Woods J, Hicks JB (1983). A plant DNA minipreparation. Plant Molecular Biotechnology Reporter 1(4):1921.

Foster JT, Allan GJ, Chan AP, Rabinowicz PD, Ravel J, Jackson PJ, Keim P (2010). Single nucleotide polymorphisms for assessing genetic diversity in castor bean (Ricinus communis). BMC Plant Biology 10(13):1-11.

Gana AK, Yusuf AF, Apuyor B (2013). Castor oil plant and its potential in transformation and industrialization of under developing nations in the world. Advanced Journal of Agricultural Research 1(5):72-79.

Golakia PR, Poshiya1 VK, Monpara BA (2015). Identification of superior donor parents for earliness through combining ability in castor. International Journal of Research in Plant Science 5(3):26-31.

India (2004). National guidelines for the conduct of tests for distinctness, uniformity and stability of castor (Ricinus communis L.). Indian Plant Authority. Retrieved 2015 April 21 from

Milani M, Dantas FV, Martins WFS (2009). Genetic divergence among castor bean genotypes by morphologic and molecular characters. Revista Brasileira de Oleaginosas e Fibrosas 13(2):61-71.

Ogunlade MO (1993). Comparative evaluation of blanket and fractional recovery fertilizer recommendation models. MSc Thesis, University of Ibadan, Nigeria.

Ogunniyi DS (2006). Castor oil: a vital industrial raw material. Bioresource Technology 97(9):1086-1091.

Qiu L, Yang C, Tian B, Yang JB, Liu A (2010). Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean (Ricinus communis L.). BMC Plant Biology 10(1):278-285.

Quintero VP, Lopez JLA, Colin CAN, Colmenero AZ, Garcia NM, Bonilla JLS, Rangel MRA (2013). Assessing the genetic diversity of castor bean from Chiapas, Mexico using SSR and AFLP markers. Industrial Crops and Products 41:134-143.

Rao PVR, Shankar VG, Reddy AV (2009). Variability studies in castor (Ricinus communis L.). Research on Crops 10(3):696-698.

Rohlf FJ (2002). Geometric morphometrics in phylogeny. In: Forey P, Macleod N (Eds). Morphology, shape and phylogenetics. Francis & Taylor, London, pp 175-193.

Rukhsar P, Patel MP, Parmar DJ, Kalola AD, Kumar S (2017). Morphological and molecular diversity patterns in castor germplasm accessions. Industrial Crops and Products 97:316-323.

Salihu BZ, Gana AK, Gbadeyan T, Alabi MB (2014). Castor oil plant (Ricinus communis L.): a potential oil crop for agribusiness in Africa. International Journal of Applied Research and Technology 3(8):29-35.

Sayama T, Hwang TY, Komatsu K, Takada Y, Takahashi M, Kato S, Ishimoto M (2011). Development and application of a whole-genome simple sequence repeat panel for high-throughput genotyping in soybean. DNA Research 18(2):107-115.

Seo K, Lee G, Ma K, Hyun D, Park Y, Jung JW, ... Lee M, (2011). Isolation and characterization of 28 polymorphic SSR loci from castor bean (Ricinus communis L.). Journal of Crop Science and Biotechnology 14(2):97-103.

Shivanna S (2008). Genetic diversity, combining ability and stability analysis of selected castor lines. PhD Thesis, University of Agricultural Sciences Bangalore, India.

Weiss EA (2000). Oil seed crops. England, Blackwell Science.

Zheng L, Qi JM, Fang PP, Su JG, Xu JT, Tao AF (2010). Genetic diversity and phylogenetic relationship of castor as revealed by SRAP analysis. Wuban Zhiwuxue 28(1):1-6.




How to Cite

SALIHU, B. Z., FALUSI, O. A., ADEPOJU, A. O., AROLU, I. W., DAUDU, O. Y., ABEJIDE, D. R., & OKE, C. O. (2019). Assessment of Genetic Diversity of Promising Castor Bean (Ricinus communis L.) Genotypes in Nigeria. Notulae Scientia Biologicae, 11(3), 467–474.



Research articles
DOI: 10.15835/nsb11310346

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