Biochemical Markers of Salt Stress in European Larch (Larix decidua)
Larix decidua, the European larch, does not grow in natural saline areas, but it can be affected by salinity either by the common practice of winter de-icing of mountain roads with NaCl, or when grown as an ornamental tree in urban areas by the use of low quality, salinised irrigation water. In the present study, the responses to salt stress of young seedlings obtained from seeds of seven Carpathian larch populations were analysed. After 30 days of treatments with 150 mM NaCl, growth and biochemical parameters were determinated. Salt stress inhibited growth of all seedlings, as shown by the relative reduction of stem length and fresh weight, and induced significant changes in the needle levels of several biochemical stress markers. Seedlings from all populations showed a marked reduction of photosynthetic pigments contents and an increase of proline and malondialdehyde (MDA) concentrations. Under salt stress, plants accumulated Na+ and Cl- in the needles, whereas K+ was maintained at a steady level. Responses of seedlings from the different populations were similar, with only small quantitative differences that did not allow the identification of more salt tolerant genotypes. However, the study revealed that several of the biochemical markers mentioned above can be suitable for the rapid and non-destructive assessment of the effects of salinity in European larch.
Adams E, Shin R (2014). Transport, signaling, and homeostasis of potassium and sodium in plants. Journal of Integrative Plant Biology 56:231-249.
Al Hassan M, Chaura J, Donat-Torres M, Boscaiu M, Vicente O (2017). Antioxidant responses under salinity and drought in three closely related wild monocots with different ecological optima. AoB Plants 9 plx009.
Al Hassan M, López-Gresa MP, Boscaiu M, Vicente O (2016 a). Stress tolerance mechanisms in Juncus: Responses to salinity and drought in three Juncus species adapted to different natural environments. Functional Plant Biology 43:949-960.
Al Hassan M, Morosan M, López-Gresa MP, Prohens J, Vicente O, Boscaiu M (2016 c). Salinity-induced variation in biochemical markers provides insight into the mechanisms of salt tolerance in common (Phaseolus vulgaris) and runner (P. coccineus) beans. International Journal of Molecular Science 17:1582.
Al Hassan M, Pacurar A, López-Gresa MP, Donat-Torres MP, Llinares JV, Boscaiu M, Vicente O (2016 b). Effects of salt stress on three ecologically distinct Plantago species. PLoS One 11(8):e0160236.
Apel K, Hirt H (2004). Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology 55:373-399.
Bartels D, Sunkar R (2005). Drought and salt tolerance in plants. Critical Reviews in Plant Sciences 24:23-58.
Bates LS, Waldren RP, Teare LD (1973). Rapid determination of free proline for water-stress studies. Plant and Soil 39:205-207.
Blainski A, Lopes GC, Palazzodemello JC (2013). Application and analysis of the Folin Ciocalteu method for the determination of the total phenolic content from Limonium brasiliense L. Molecules 18:6852-6865.
Bonet A, Lelu-Walter MA, Faugeron C, Gloaguen V, Saladin G (2016). Physiological responses of the hybrid larch (Larix × eurolepis Henry) to cadmium exposure and distribution of cadmium in plantlets. Environmental Science and Pollution Research International 23:8617-8626.
Boyer JS (1982). Plant productivity and environment. Science 218:443-448.
Cicevan R, Al Hassan M, Sestras AF, Prohens J, Vicente O, Sestras RE, Boscaiu M (2016). Screening for drought tolerance in cultivars of the ornamental genus Tagetes (Asteraceae) PeerJ 4:e2133.
Clancy KM, Wagner MR, Reich PB (1995). Ecophysiology and insect herbivory. In: Smith WK, Hinckley TM (Eds). Ecophysiology of coniferous forests. Academic Press, San Diego, CA, USA pp 125-180.
Croser C, Renault S, Franklin J, Zwiazek J (2001). The effect of salinity on the emergence and seedling growth of Picea mariana, Picea glauca and Pinus banksiana. Environmental Pollution 115:9-16.
Del Rio D, Stewart AJ, Pellegrini N (2005). A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutritional, Metabolic and Cardiovascular Diseases 15:316-328.
Ditmarová L, Kurjak D, Palmroth S, Kmet J, Strelcová K (2010). Physiological responses of Norway spruce (Picea abies) seedlings to drought stress. Tree Physiology 30:205-213.
Dubois M, Gilles KA, Hamilton JK, Reberd PA, Smith F (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350-356.
Fini A, Brunetti C, Di Ferdinando M, Ferrini F, Tattini M (2011). Stress-induced flavonoid biosynthesis and the antioxidant machinery of plants. Plant Signaling and Behavior 6:709-711.
Flowers TJ, Colmer TD (2015). Plant salt tolerance: adaptations in halophytes. Annals of Botany 115:327-331.
Flowers TJ, Colmer TD (2008). Salinity tolerance in halophytes. New Phytologist 179:945-963.
Geburek T (2010). Larix decidua Miller, 1768. In: Roloff A, Weissgerber H, Lang U, Stimm B (Eds). Bäume Mitteleuropas Wiley, Weinheim, Germany pp 431-450.
Gierth M, Mäser P (2007). Potassium transporters in plants- Involvement in K+ acquisition, redistribution and homeostasis. FEBS Letters 581:2348-2356.
Goodrich BA, Koski RD, Jacobi WR (2009). Condition of soils and vegetation along roads treated with magnesium chloride for dust suppression. Water, Air and Soil Pollution 198:165-188.
Gramazio P, Plesa IM, Truta AM, Sestras AF, Vilanova S, Plazas M, Vicente O, Boscaiu M, Prohens J, Sestras RE (2018). Highly informative SSR genotyping reveals large genetic diversity and limited differentiation in European larch (Larix decidua) populations from Romania. Turkish Journal of Agriculture and Forestry 42:165-175.
Greenway H, Munns R (1980). Mechanisms of salt tolerance in non-halophytes. Annual Review of Plant Biology 31:149-190.
Hoagland DR, Arnon DI (1950). The water-culture method for growing plants without soil. California Agricultural Experiment Station Publications Series, College of Agriculture, University of California Davis, CA, USA.
Hodges DM, Delong JM, Forney CF, Prange RK (1999). Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604-611.
Kayama M, Quoreshi AM, Kitaoka S, Kitahashi Y, Sakamoto Y, Maruyama Y, Kitao M, Koike T (2003). Effects of deicing salt on the vitality and health of two spruce species, Picea abies Karst., and Picea glehnii Masters planted along roadsides in northern Japan. Environmental Pollution 124:127-137.
Koskela J, Buck A, du Cros ET (2007). EUFORGEN Climate change and forest genetic diversity: Implications for sustainable forest management in Europe; Biodiversity International 2007. Retrieved 2017 November 15, from http://www.euforgen.org/fileadmin/ bioversity/publications/ pdfs/1216.
KoÅºmiÅ„ska A, Al Hassan M, Hanus-Fajerska E, Naranjo MA, Boscaiu M, Vicente O (2018). Comparative analysis of water deficit and salt tolerance mechanisms in Silene. South African Journal of Botany 117:193-206.
KoÅºmiÅ„ska A, Al Hassan M, Wiszniewska A, Hanus-Fajerska E, Boscaiu M, Vicente O (2019). Response of succulents to drought: Comparative analysis of four Sedum (Crassulaceae) species. Scientia Horticulturae 243:235-242.
Kumar D, Al Hassan M, Naranjo MA, Agrawal V, Boscaiu M, Vicente O (2017). Effects of salinity and drought on growth, ionic relations, compatible solutes and activation of antioxidant systems in oleander (Nerium oleander L.). PLoS One 12(9):e0185017.
Lichtenthaler HK, Wellburn AR (1983). Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions 11:591-592.
Lindner M, Maroschek M, Netherer S, Kremer A, Barbati A, Garcia-Gonzalo J, Lexer MJ (2010). Climate change impacts, adaptive capacity, and vulnerability of European forest ecosystems. Forest Ecology and Management 259:698-709.
Matras J, Pâques L (2008). EUFORGEN Technical guidelines for genetic conservation and use for European Larch (Larix decidua). Biodiversity International, Rome, Italy.
Mbarki S, Sytar O, Cerda A, Zivcak M, Rastogi A, He X, Zoghlami A, Abdelly C, Brestic M (2018). Strategies to mitigate the salt stress effects on photosynthetic apparatus and productivity of crop plants. In: Kumar V, Wani SH, Suprasanna P, Trann LSP (Eds). Salinity responses and tolerance in plants, Volume 1. Targeting Sensory, Signaling Mechanisms. Springer International Publishing AG, Cham, Switzerland pp 85-136.
Munns R, Tester M (2008). Mechanisms of salinity tolerance. Annual Reviews in Plant Biology 59:651-681.
Newton RJ, Sen S, Puryear JD (1986). Free proline changes in Pinus taeda L. callus in response to drought stress. Tree Physiology 1:325-332.
Nxele X, Klein A, Ndimba BK (2017). Drought and salinity stress alters ROS accumulation, water retention, and osmolyte content in sorghum plants. South African Journal of Botany 108:261-266.
Plesa IM, González-Orenga S, Al Hassan M, Sestras AF, Vicente O, Prohens J, Sestras RE, Boscaiu M (2018). Effects of drought and salinity on European Larch (Larix decidua Mill.) seedlings. Forests 9:320.
Renault S (2005). Tamarack response to salinity: Effects of sodium chloride on growth and ion, pigment, and soluble carbohydrate levels. Canadian Journal of Forest Research 35:2806-2812.
Rodriguez-Navarro A, Rubio F (2006). High-affinity potassium and sodium transport systems in plants. Journal of Experimental Botany 57:1149-1160.
Santos CV (2004). Regulation of chlorophyll biosynthesis and degradation by salt stress in sunflower leaves. Scientia Horticulturae 103:93-99.
Shiop ST, Al Hassan M, Sestras AF, Boscaiu M, Sestras RE, Vicente O (2015). Identification of salt stress biomarkers in Romanian Carpathian populations of Picea abies (L.) Karst. PLoS One 10:e0135419.
Schiop ST, Al Hassan M, Sestras AF, Boscaiu M, Sestras RE, Vicente O (2017). Biochemical responses to drought, at the seedling stage, of several Romanian Carpathian populations of Norway spruce (Picea abies (L.) Karst). Trees 31:1479-1490.
Szabados L, Savouré A (2010). Proline: A multifunctional aminoacid. Trends in Plant Science 15:89-97.
Taïbi K, del Campo AD, Vilagrosa A, Bellés JM, López-Gresa MP, Pla D, Calvete JJ, López-Nicolás JM, Mulet JM (2017). Drought tolerance in Pinus halepensis seed sources as identified by distinctive physiological and molecular markers. Frontiers in Plant Science 8:202.
Várallyay G (2010). The impact of climate change on soils and on their water management. Agronomy Research 8:385-396.
Verbruggen N, Hermans C (2008). Proline accumulation in plants: A review. Amino Acids 35:753-759.
Weimberg R (1987). Solute adjustments in leaves of two species of wheat at two different stages of growth in response to salinity. Physiologia Plantarum 70:381-388.
Zamani M, Hakimi MH, Mosleh Arany A, Kiani B, Rashtian A (2014). Comparing the effects of SNP and SA under salinity stress on proline, sugar, Na, K and chlorophyll of leaves of Pinus eldarica and Cupressus sempervirens in Iran. Bulletin of Environment Pharmacology and Life Sciences 3:91-95.
Zhishen J, Mengcheng T, Jianming W (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64:555-559.
Zhu JK (2001). Plant salt tolerance. Trends in Plant Science 6:66-71.
Zhu JK (2007). Plant salt stress. Encyclopaedia of Life Sciences. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0001300.pub2].
Zhu JK (2016). Abiotic stress signalling and responses in plants. Cell 167:313-324.
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