Effects of Prior Heat Stress on the Growth and Phytochemical Contents Accumulation of Amaranthus hybridus (Linn.)


  • Ezekiel Dare OLOWOLAJU Obafemi Awolowo University, Faculty of Science, Department of Botany, Ile-Ife (NG)
  • Gideon O. OKUNLOLA Osun State University, Faculty of Science, Department of Biological Sciences, Osogbo (NG)
  • Abiodun M. ADEJUMO Obafemi Awolowo University, Faculty of Science, Department of Botany, Ile-Ife (NG)
  • Adekunle A. ADELUSI Obafemi Awolowo University, Faculty of Science, Department of Botany, Ile-Ife (NG)




biochemical; early stage; growth; heat; phytochemicals; stress


The present study aimed at investigating the impact of abrupt heat stress on growth and phytochemical contents accumulation in Amaranthus hybridus. The treatments were as follows: control without heat treatment, seedlings subjected to heat at 45 oC for two hours and seedlings subjected to heat at 45 oC for four hours. After the stipulated time for each category, plants were removed from the Gallenkamp oven and were transplanted into other sets of thirty six pots (of 21 cm deep and 24 cm in diameter), as well as the control. The seedlings were kept in a screen house to minimise extraneous factors such as pests and rodents. They were watered daily with 200 mL of tap water in the morning and 200 mL of tap water in the evening until they were fully established. The phytochemical contents were determined at vegetative, flowering and fruiting stage using ethanolic extracts from the dried leaves of plant samples. From the results obtained, it was observed that leaf, shoot and root fresh and dry weights of the stressed plants were lower than the control plants. Exposure of the plants at different durations of heat treatment enhanced and inhibits the quantities of phytochemicals at different growth stages. From the present study it can be concluded that heat stress, on the basis of global warming in the future, will likely have overall negative effects on the growth of Amaranthus hybridus that will become more severe as the time of exposure increases and and might cause variation in the level of phytochemical constituents of Amaranthus hybridus at different growth stages.


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Bray EA, Bailey-Serres J, Weretilnyk E (2000). Responses to abiotic stresses. In: Gruissem W, Buchannan B, Jones R (Eds). Biochemistry and Molecular Biology of Plants. American Society of Plant Physiologists, Rockville, MD pp 1158-1249.

Chaves MM, Maroco JP, Pereira JS (2003). Understanding plant responses to drought from gene to the whole plant. Functional Plant Biology 30(3):239-289.

Edeoga HO, Okwu DE, Mbaebie BO (2005). Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology 4(7):685-688.

Firn R (2010). Nature chemicals. Oxford University Press pp 74-75.

Giri A (2013). Effect of acute heat stress on nutrient uptake by plant roots. Theses and Dissertations. The University of Toledo, pp 32.

He HP, Corke H, Cai JG (2003). Supercritical carbon dioxide extraction of oil and squalene from Amaranthus grain. Journal of Agricultural and Food Chemistry 51(27):7921-7925.

Heckathorn SA, Giri A, Mishra S, Bista D (2013). Heat stress and roots. In N.T.A. S. Gill (Ed), Climate Change. Germany: Wiley-VCH.

Knekt P, Jarvinen R, Seanen M, Heliovaara L, Aromaa A (1997). Dietary flavonoids and the risk of lung cancer and other malignant neoplasms. American Journal of Epidemiology 146:223-230.

Liu X, Huang B (2000). Heat stress injury in relation to membrane lipid peroxidation in creeping bentgrass. Crop Science 40(2):503-510.

Liu X, Huang B (2002). Cytokinin effects on creeping bentgrass response to heat stress. Crop Science 42(2):466-472.

Mainali KP (2007). Impact of heat waves on plant-soil links in tall-grass prairie. The University of Toledo.

Mirza H, Kamrun F, Masavuki F (2003). Extreme temperature responses, oxidative and antioxidant defense in plants. In: Abiotic stress - Plant responses and applications in agriculture. InTech.

Muuns R, James RA, Lauchli A (2006). Approaches to increasing the heat tolerance of wheat and other cereals. Journal of Experimental Botany 57:645-663.

Rao CV, Newmark HL (1998). Chemo-revenive effect of squalene on colon cancer. Carcinogenesis. Journal of Medicinal Plants 19:287-290.

Wahid A, Close TJ (2007). Expression of dehydrin under heat stress and their relationship with water relations of sugarcane leaves. Biologia Plantarum 51:104-109.

Zhang W, Wang SY (2000). Antioxidant activity and phenolic compounds in selected herbs. Journal of Agriculture and Food Chemistry 49(11):5165-5170.

Zhu JK (2001). Genetic analysis of plant salt tolerance using Arabidopsis. Plant Physiology 124: 941-948.




How to Cite

OLOWOLAJU, E. D., OKUNLOLA, G. O., ADEJUMO, A. M., & ADELUSI, A. A. (2018). Effects of Prior Heat Stress on the Growth and Phytochemical Contents Accumulation of Amaranthus hybridus (Linn.). Notulae Scientia Biologicae, 10(2), 240–244. https://doi.org/10.15835/nsb10210270



Research articles
DOI: 10.15835/nsb10210270