Effects of Fertilizer Application and Successive Harvesting on Clipping Yield, Phytochemical Contents and Antioxidant Activity of Cynodon dactylon (L.) Pers.

  • Vella Ah Chon FUNG University Malaysia Sabah, Faculty of Sustainable Agriculture, Locked Bag No.3, 90509 Sandakan, Sabah (MY)
  • Januarius GOBILIK University Malaysia Sabah, Faculty of Sustainable Agriculture, Locked Bag No.3, 90509 Sandakan, Sabah (MY)
  • Devina DAVID University Malaysia Sabah, Faculty of Sustainable Agriculture, Locked Bag No.3, 90509 Sandakan, Sabah (MY)
Keywords: Bermuda grass; cutting frequency; nitrogen rate; organic/ inorganic fertilizer


The present study aimed to investigate the effects of fertilizer application and successive harvesting on phytochemical contents and antioxidant activities of Cynodon dactylon, a medicinal Bermuda grass in Sabah (Malaysia). Three fertilizers of two nitrogen concentrations were used in the experiment. The grass was harvested successively three times at five-weeks interval. Grass treated with 25 kg N/ha/month from the first harvest was found to have the highest clipping yield. Successive harvesting decreased the dry matter production of the grass irrespective of N concentration applied. Total saponin and alkaloid contents of the grass were increased by a combination treatment of fertilizer type ´ rate ´ harvesting; total flavonoid content was increased by fertilizer type ´ harvesting treatments; however, total phenolic content was not affected by any of the treatment or combination of the treatments. Both of the antioxidant assays (DPPH and FRAP) indicated that antioxidant activity of the grass was increased by fertilizer rate ´ harvesting treatments. There was a significant correlation found between total phenolic and flavonoid contents and antioxidant activities, suggesting that these two secondary metabolites may contribute to the antioxidant property of the grass. Overall, the obtained data indicated that the described treatments could be used to manipulate the production and accumulation of bioactive compounds of C. dactylon.


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Abdullah SY, Gobilik JA, Chong KP (2012). Preliminary phytochemical study and antimicrobial activity from various extract of Cynodon dactylon (L.) Pers. (Bermuda) against selected pathogens. International Journal of Pharmacy and Pharmaceutical Sciences 4(5):227-230.

Al-Humaid AI (2005). Effects of compound fertilization on growth and alkaloids of Datura plants. Journal of Plant Nutrition 27(12):2203-2219.

Aldous DE, Wilson JR (1999). International Turf Management, Routledge.

Amaglo NK, Timpo GM, Ellis WO, Bennett RN (2007). Effect of spacing and harvest frequency on the growth and leaf yield of moringa (Moringa oleifera Lam.), a leafy vegetable crop. Ghana Journal of Horticulture 6:33-40.

Barroso, MR, Martins N, Barros L, Antonio AL, Rodrigues MÂ, Sousa MJ, Santos-Buelga C, Ferreira ICFR (2018). Assessment of the nitrogen fertilization effect on bioactive compounds of frozen fresh and dried samples of Stevia rebaudiana Bertoni. Food Chemistry 243:208-213.

Benzie IF, Strain JJ (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry 239(1):70-76.

Brasileiro BG, Leite JPV, Casali VWD, Pizziolo VR, Coelho OGL (2015). The influence of planting and harvesting times on the total phenolic content and antioxidant activity of Talinum triangulare (Jacq.) Willd. Acta Scientiarum. Agronomy 37(2):249-255.

Chandel E, Kumar B (2015). Antimicrobial activity and phytochemical analysis of Cynodon dactylon: A review. Journal of Pharmacy and Pharmaceutical Sciences 4(11):515-530.

Dixon RA, Paiva NL (1995). Stress-induced phenylpropanoid metabolism. The Plant Cell 7(7):1085-1097.

Farshchi HSK, Arani MA, Nemati SH (2014). Phytochemical and morphological attributes of St. John's wort. Notulae Scientia Biologicae 6(3):326-330.

Fernandez-Panchon MS, Villano D, Troncoso AM, Garcia-Parrilla MC (2008). Antioxidant activity of phenolic compounds: from in vitro results to in vivo evidence. Critical Review in Food Science and Nutrition 48(7):649-671.

Fook YC, Kheng YS (2009). Antioxidative and antibacterial activities of Pangium edule seed extracts. International Journal of Pharmacology 5(5):285-297.

Gholamhosseinpour Z, Hemati K, Dorodian H, Bashiri-Sa Z (2011). Effect of nitrogen fertilizer on yield and amount of alkaloids in periwinkle and determination of Vinblastine and Vincristine by HPLC and TLC. Plant Sciences Research 3(2):4-9.

Gobilik J, Jerome V, David D (2013). Preliminary selection of some ecotypes of Cynodon dactylon (L.) Pers. in Sabah, Malaysia, for turfgrass use. Journal of Tropical Biology and Conservation 10:51-66.

Gull J, Sultana B, Anwar F, Naseer R, Ashraf M, Ashrafuzzaman M (2012). Variation in antioxidant attributes at three ripening stages of guava (Psidium guajava L.) fruit from different geographical regions of Pakistan. Molecules 17(3):3165-3180.

Hallmann E, Rembiałkowska E (2012). Characterisation of antioxidant compounds in sweet bell pepper (Capsicum annuum L.) under organic and conventional growing systems. Journal of the Science of Food and Agriculture 92(12):2409-2415.

Hassan SA, Mijin S, Yusoff UK, Ding P, Wahab PEM (2012). Nitrate, ascorbic acid, mineral and antioxidant activities of Cosmos caudatus in response to organic and mineral-based fertilizer rates. Molecules 17(7):7843-7853.

Ibrahim M H, Jaafar HZE, Karimi E, Ghasemzadeh A (2013). Impact of organic and inorganic fertilizers application on the phytochemical and antioxidant activity of Kacip Fatimah (Labisia pumila Benth). Molecules 18(9):10973-10988.

Ilupeju EAO, Akanbi WB, Olaniyi JO, Lawal BA, Ojo MA, Akintokun PO (2015). Impact of organic and inorganic fertilizers on growth, fruit yield, nutritional and lycopene contents of three varieties of tomato (Lycopersicon esculentum (L.) Mill) in Ogbomoso, Nigeria. African Journal of Biotechnology 14(31):2424-2433.

Jacobo-Velázquez DA, González-Agüero M, Cisneros-Zevallos L (2015). Cross-talk between signalling pathways: the link between plant secondary metabolite production and wounding stress response. Scientific Reports 5:8608.

Kedare SB, Singh RP (2011). Genesis and development of DPPH method of antioxidant assay. Journal of Food Science and Technology 48(4):412-422.

Khlifi D, Hayouni EA, Valentin A, Cazaux S, Moukarzel B, Hamdi M, Bouajila J (2013). LC–MS analysis, anticancer, antioxidant and antimalarial activities of Cynodon dactylon L. extracts. Industrial Crops and Products 45:240-247.

Manyawu GJ, Chakoma C, Sibanda S, Mutisi C, Chakoma IC (2003). The effect of harvesting interval on herbage yield and nutritive value of Napier grass and hybrid Pennisetums. Asian Australasian Journal of Animal Sciences 16(7):996-1002.

Mary JAL, Nithiya T (2015). Effect of organic and inorganic fertilizer on growth, phenolic compounds and antioxidant activity of Solanum nigrum . L. Journal of Pharmacy and Pharmaceutical Sciences 4(5):808-822.

Mohadjerani M, Tavakoli R, Hosseinzadeh R (2014). Fatty acid composition, antioxidant and antibacterial activities of Adonis wolgensis L. extract. Avicenna Journal of Phytomedicine 4(1):24.

Murali KS, Sivasubramanian S, Vincent S, Murugan SB, Giridaran B, Dinesh S, Sathishkumar R (2015). Anti-chikungunya activity of luteolin and apigenin rich fraction from Cynodon dactylon. Asian Pacific Journal of Tropical Medicine 8(5):352-358.

Nagori BP, Solanki R (2011). Cynodon dactylon (L.) Pers.: A valuable medicinal plant. Research Journal of Medicinal Plants 5(5):508-514.

Naguib AEM M, El-Baz FK, Salama ZA, Abd El Baky Hanaa H, Ali HF, Gaafar AA (2012). Enhancement of phenolics, flavonoids and glucosinolates of broccoli (Brassica olaracea, var. Italica) as antioxidants in response to organic and bio-organic fertilizers. Journal of the Saudi Society of Agricultural Sciences 11(2):135-142.

Onofrei V, Burducea M, Lobiuc A, Teliban GC, Ranghiuc G, Robu T (2017). Influence of organic foliar fertilization on antioxidant activity and content of polyphenols in Ocimum basilicum L. Acta Poloniae Pharmaceutica 74(2):611-615.

Onyeonagu CC, Ukwueze CC (2012). Anti-nutrient components of guinea grass (Panicum maximum) under different nitrogen fertilizer application rates and cutting management. African Journal of Biotechnology 11(9):2236-2240.

Petropoulos S, Fernandes Â, Karkanis A, Ntatsi G, Barros L, Ferreira IC (2017). Successive harvesting affects yield, chemical composition and antioxidant activity of Cichorium spinosum L. Food Chemistry 237:83-90.

Rivai RR, Wardani FF, Zulkarnaen RN (2017). The effect of NPK fertilizer and planting media on plant growth and saponin content of the medicinal plant Anchomanes difformis. Nusantara Bioscience 9(2):141-145.

Salminen SO, Grewal PS, Quigley MF (2003). Does mowing height influence alkaloid production in endophytic tall fescue and perennial ryegrass? Journal of Chemical Ecology 29(6):1319-1328.

Singleton VL, Orthofer R, Lamuela-Raventós RM (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology 299:152-178.

Shamsa F, Monsef H, Ghamooshi R, Verdian-rizi M (2008). Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Thai Journal of Pharmaceutical Science 32:17-20.

Sudha A, Srinivasan P (2013). Physicochemical and phytochemical profiles of aerial parts of Lippia nodiflora L. International Journal of Pharmaceutical Sciences and Research 4(11):4263.

Tavarini S, Sgherri C, Ranieri AM, Angelini LG (2015). Effect of nitrogen fertilization and harvest time on steviol glycosides, flavonoid composition, and antioxidant properties in Stevia rebaudiana Bertoni. Journal of Agricultural and Food Chemistry 63(31):7041-7050.

Turgeon AJ (2008). Turfgrass Management. (8th ed). Upper Saddle River: Pearson Education International.

Vashisth T, Olmstead, MA, Olmstead J, Colquhoun TA (2017). Effects of nitrogen fertilization on subtropical peach fruit quality: organic acids, phytochemical content, and total antioxidant capacity. Journal of the American Society for Horticultural Science 142(5):393-404.

Vinther FP (2006). Effects of cutting frequency on plant production, Nâ€uptake and N2 fixation in aboveâ€and belowâ€ground plant biomass of perennial ryegrass–white clover swards. Grass and Forage Science 61(2):154-163.

War AR, Paulraj MG, Ahmad T, Buhroo AA, Hussain B, Ignacimuthu S, Sharma HC (2012). Mechanisms of plant defense against insect herbivores. Plant Signalling and Behaviour 7(10):1306-1320.

Wong CC, Li HB, Cheng KW, Chen F (2006). A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chemistry 97(4):705-711.

Xia P, Guo H, Zhao H, Jiao J, Deyholos MK, Yan X, Liu Y, Liang Z (2016). Optimal fertilizer application for Panax notoginseng and effect of soil water on root rot disease and saponin contents. Journal of Ginseng Research 40:38-46.

Zhang X, Li C, Nan Z (2011). Effects of cutting frequency and height on alkaloid production in endophyte-infected drunken horse grass (Achnatherum inebrians). Science China Life Sciences 54(6):567-571.

Zou Y, Liao S, Shen W, Liu F, Tang C, Chen CYO, Sun, Y (2012). Phenolic and antioxidant activity of mulberry leaves depend on cultivar and harvest month in Southern China. International Journal of Molecular Sciences 13(12):16544-16553.

How to Cite
FUNG, V. A. C., GOBILIK, J., & DAVID, D. (2018). Effects of Fertilizer Application and Successive Harvesting on Clipping Yield, Phytochemical Contents and Antioxidant Activity of Cynodon dactylon (L.) Pers. Notulae Scientia Biologicae, 10(1), 130-136. https://doi.org/10.15835/nsb10110226
Research articles
DOI: 10.15835/nsb10110226