Litsea glutinosa (Lauraceae): Evaluation of its Foliar Phytochemical Constituents for Antimicrobial Activity
DOI:
https://doi.org/10.15835/nsb10110180Keywords:
extract; Litsea glutinosa; microorganism; pathogen; phytochemicalAbstract
The phytochemical investigation of the leaves of Litsea glutinosa revealed the presence of secondary metabolites like alkaloids, anthraquinones, cardiac glycosides, flavonoids, glycosides, phenols, saponins, steroids, tannins, terpenoids, volatile compounds, amino acids and carbohiydrates. The antimicrobial activity and minimum inhibition concentration values were determined for these phytochemical constituents as crude extracts using the agar well diffusion and two-fold serial dilution methods. The results indicated that Bacillus subtilis was the most susceptible bacterium with high inhibition zones for the methanol and chloroform extracts of 31 mm and 26 mm, respectively. The MIC values indicated that extracts possess good antimicrobial activity with significant MIC value against Enterococcus faecalis, Pseudomonas aeruginosa and Staphylococcus pneumoniae at 31.2 μg/ml concentrations. The extracts showed marked antimicrobial activity against both bacteria and fungi. Among the bacterial strains, gram-positive bacteria were more susceptible than the gram-negative. All the 13 microorganisms tested showed dose dependent susceptibility towards the phytochemicals present in the foliar extracts. The study suggests that Litsea glutinosa leaves possess potent antimicrobial activity and can be a good source for the development of new antibiotics.
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Agrawal N, Choudhary AS, Sharma MC, Dobhal MP (2011). Chemical constituents of plants from the genus Litsea. Chemistry & Biodiversity 8:223-243.
Ali MA, Sayeed MA, Bhuiyan MSA, Sohel FI, Yeasmin MS (2004). Antimicrobial screening of Cassia fistula and Mesua ferrea. Journal of Medical Sciences 4:24-29.
Aniel Kumar O, Krishna Rao M, Mutyala Naidu L (2014). Evaluation of antimicrobial activity of chemical constituents of Achyranthes aspera L. roots against human pathogens. Indian Journal of Natural Products and Resources 5(3):278-281.
Aniel Kumar O, Krishna Rao M, Mutyala Naidu L (2015). Evaluation of antimicrobial activity of root extracts of Abutilon indicum. Notulae Scientia Biologicae 7(2):160-163.
Aniel Kumar O, Mutyala Naidu L, Raja Rao KG (2010). Antibacterial evaluation of snake weed (Euphorbia hirta L.). Journal of Phytology 2(3):08-12.
Anonymous (2000). Centers for Disease Control and Prevention, Preventing emerging infectious diseases, a strategy for the 21st century.
Chang SY, Cheng MJ, Kuo YH, Lee SJ, Chen IS (2008). Secondary metabolites from the stem bark of Litsea akoensis and their cytotoxic activity. Helvetica Chimica Acta 91(6):1156-1165.
Cheng MJ, Wang TA, Lee SJ, Chen IS (2010). A new butanolide and a new secobutanolide from Litsea lii var. nunkao-tahangensis. Natural Product Research 24(7):647-656.
Chowdhury JU, Bhuiyan NI, Nandi NC (2008). Aromatic plants of Bangladesh: Essential oils of leaves and fruits of Litsea glutinosa (Lour.) C.B. Robinson. Bangladesh Journal of Botany 37(1):81-83.
Devi P, Meera R (2010). Study of antioxidant, anti-inflammatory and wound healing activity of extracts of Litsea glutinosa. Journal of Pharmaceutical Science and Research 2(2):155-163.
Feng T, Zhang RT, Tan QG, Zhang XY, LiuYP, Cai XH (2009). Two new isoquinoline alkaloids from Litsea cubeba. Zeitschrift für Naturforschung B 64:871-874.
Gulzar H, Chowdhury M, Alam MN, Alam S, Shakil M, Khan S, Rahman M, Mazumdar M (2015). Phytochemical screening, antimicrobial and anticancerous activities of two different plant extracts. Journal of Medicinal Plants Studies 3(6):76-81.
Guzman GSI, Navarrete A (2009). Pharmacological exploration of the sedative mechanism of hesperidin indentified as the active principle of Citrus sinensis flowers. Planta Medica 75:295-301.
Haque T, Uddin MZ, Saha M, Mazid A, Hassan A (2014). Propagation, antibacterial activity and phytochemical profiles of Litsea glutinosa (Lour.) C.B. Robinson. Dhaka University Journal Biological Sciences 23(2):165-171.
Hosseinzadeh M, Mohamad J, Khalilzadeh MA, Zardoost MR, Haak J, Rajabi M (2013). Isolation and characterization of bioactive compounds from the bark of Litsea costalis. Journal of Photochemistry Photobiology B 128:85-91.
Jia XJ, Dong L, Yang Y, Yuan S, Zhang ZW, Yuan M (2013). Preliminary structural characterization and antioxidant activities of polysaccharides extracted stem Hawk tea (Litsea coreana var. lanuginose). Carbohydr Polymers 95:195-199.
Lohitha P, Shivsagar K, Charan V, Priya UP, Sagar SV, Ramanjaneyulu K, Verma V (2010). Phytochemical screening and evaluation of in vitro antibacterial activity of Litsea glutinosa (L) bark ethanol extract. Pharmacologyonline 1:618-623.
Mandal SC, Kumar CK, Majumder A, Majumder R, Maity BC (2000). Antibacterial activity of Litsea glutinosa bark. Fitoterapia 71(4):439-441.
Meera R, Devi P (2009). Antimicrobial activity of methanolic extract of Merrimea gagentica and Litsea glutinosa Leaves. Asian Journal of Chemistry 21(7):5655-5660.
Ncube NS, Afolayan AJ, Okoh AL (2008). Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. African Journal of Biotechnology 7(12):1797-1806.
Pan JY, Zhang S, Jun W, Li QX, Xiao ZH (2010). Litseaglutinan A and lignans from Litsea glutinosa. Helvetica Chimica Acta 93(5):951-957.
Poornima VH (2011). Evaluation of antimicrobial activity of Litsea glutinosa. International Journal of Pharmaceutical Applications 2(1):105-114.
Rabena AR (2010). Propagation techniques of endangered sablot (Litsea glutinosa) Lour. C.B. Robinson. JPAIR Multidisciplinary Journal 5:56-83.
Silva KA, Klein Junior LC, Cruz SM, Caceres A, Quintao N, Monache FD (2012). Anti-inflammatory and anti-hyperalgesic evaluation of the condiment laurel (Litsea guatemalensis Mez.) and its chemical composition, Food Chemistry 132(4):1980-1986.
Thomas RP, Antony AM, Mamen AA (2013). Comparative phytochemical analysis of Diospyros chloroxylon leaves in various extracts. International Journal of Scientific and Research Publications 3(9):1-4.
Wang H, Liu Y (2010). Chemical composition and antibacterial activity of essential oils from different parts of Litsea cubeba. Chem Biodivers 7:229-235.
Wang YS, Huang R, Lu H, Li F, Yang JH (2010). A new 2’-oxygenated flavones glycoside from Litsea glutinosa (Lour.) C.B. Rob. Biosciences Biotechnology Biochemistry 74(3):652-654.
Wang YS, Zhen L, Yan L, Rong H, Hong BZ, Jing Y (2011). A new Megastigmane Diglycoside from Litsea glutinosa (Lour.) C. B. Rob. Journal of Brazilian Chemical Society 22(11):2234-2238.
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