Molecular Level Investigation of Staphylococci’s Resistance Mechanisms to Antibiotics

  • Lavinia Lorena PRUTEANU 1# Current Address: Centre for Molecular Science Informatics, Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK; 2 Institute for Doctoral Studies, Babeș-Bolyai University, Kogălniceanu Street no. 1, 400084 Cluj-Napoca; 3 University of Medicine and Pharmacy "Iuliu Hațieganu", MedFuture, Research Center for Advanced Medicine, 23 Gh. Marinescu str./4-6 Pasteur str, 400337, Cluj-Napoca (RO)
  • Lorentz JÄNTSCHI 1 Institute for Doctoral Studies, Babeș-Bolyai University, Kogălniceanu Street no. 1, 400084 Cluj-Napoca; 2 Technical University of Cluj-Napoca, Department of Physics and Chemistry, Muncii Blvd. no. 103-105, 400641 Cluj-Napoca (RO)
  • Cornelia CRĂCIUNAȘ Babeș-Bolyai University, Faculty of Biology and Geology, Department of Experimental Biology, 5-7 Clinicilor, 400006 Cluj-Napoca (RO)
Keywords: bacterial resistance to antibiotics; beta-lactams; molecular level investigation; pathogenic activity genes; Staphylococcus bacteria

Abstract

Polymerase chain reaction (PCR ) techniques development allows elaboration of many assays for identification of bacteria’s resistance mechanisms to antibiotics.  Following this idea, the results of molecular level investigation of bacteria’s resistance mechanisms to antibiotics may give many opportunities to find more rapid methods for identifying the genes which are responsible for antibiotic resistance induction. The aim of this study was to investigate antibiotic resistance genes in Staphylococcus bacteria on molecular level. As classes of antibiotics it was used macrolides-lincosamides-streptogramin B (MLSB) and beta-lactams. In the proposed study the bacterial strains are represented by 50 isolates of Staphylococcus. The bacterial strains were analyzed using polymerase chain reaction to identify the nuc, tuf, tst, sea, pathogenic activity genes. After this, the bacteria were tested for ermA, ermB, ermC genes and for mecA, femA which are involved in resistance to macrolides, lincosamides, streptogramin B and to beta-lactams, respectively. The presence or the absence of these genes confirms that tested strains are resistant to specific antibiotic or not. Bacteria pathogenic activity was emphasized by genes as follows: sea (enterotoxin) which was found at all isolates, tst (toxic shock toxin) gene was not detected in any of isolates and tuf gene (elongation factor) was obtained with one pair of primers. Resistance to beta-lactams was evidenced by the presence of mecA in all isolates and femA in some strains. Each of ermC, ermA and ermB, macrolides-lincosamides-streptogramin B resistance genes, were detected.

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Published
2017-09-30
How to Cite
PRUTEANU, L. L., JÄNTSCHI, L., & CRĂCIUNAȘ, C. (2017). Molecular Level Investigation of Staphylococci’s Resistance Mechanisms to Antibiotics. Notulae Scientia Biologicae, 9(3), 307-314. https://doi.org/10.15835/nsb9310153
Section
Research articles
CITATION
DOI: 10.15835/nsb9310153