Production of Phytotoxic Metabolite Using Biphasic Fermentation System from Strain C1136 of Lasiodiplodia pseudotheobromae, a Potential Bioherbicidal Agent

  • Charles Oluwaseun ADETUNJI Landmark University, Department of Microbiology, Laboratory of Microbiology, Biotechnology and Nanotechnology, Omu-Aran
  • Julius Kola OLOKE Ladoke Akintola University of Technology, Department of Pure and Applied Biology, P.M.B 4000, Ogbomoso, Oyo State
  • Gandham PRASAD Institute of Microbial Technology, Department of Molecular Biology and Microbial Type Culture and Gene Bank (MTTC), Sector 39A ,Chandigarh
  • Moses ABALAKA Federal University of Technology, Department of Microbiology, Minna, Niger State,
  • Emenike Onyebum IROKANULO Landmark University, Department of Microbiology, Laboratory of Microbiology, Biotechnology and Nanotechnology, Omu-Aran

Abstract

Formulation of effective and environmental friendly bioherbicides depends on the type of fermentation medium used for the production of phytotoxic metabolites. The effect of biomass, colony forming unit and the phytotoxic metabolite produced from the biphasic fermentation was carried out, while the phytotoxic metabolite was  tested in vivo and in-vitro on Echinochola crus-galli and dicotyledonous Chromolaena odorata. The mutant strain of Lasiodiplodia pseudotheobromae C1136 (Lp90) produced the highest amount of conidia and the largest necrotic area on the two tested weeds when compared to its wild strain in the different biphasic media combinations. The study revealed that the biphasic system containing PDB + rice produced the highest bioherbicidal activities. Therefore, the phytotoxic metabolites from strain C1136 are suggested for large scale production of bioherbicides for the management of weeds in conventional farming to improve yield and enhance food security.

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Published
2017-09-30
How to Cite
ADETUNJI, C. O., OLOKE, J. K., PRASAD, G., ABALAKA, M., & IROKANULO, E. O. (2017). Production of Phytotoxic Metabolite Using Biphasic Fermentation System from Strain C1136 of Lasiodiplodia pseudotheobromae, a Potential Bioherbicidal Agent. Notulae Scientia Biologicae, 9(3), 371-377. https://doi.org/10.15835/nsb9310082
Section
Research articles