Detection of Extended Spectrum Beta-Lactamases Among Gram Negative Bacilli Recovered from Cattle Feces In Benin City, Nigeria

  • Helen Oroboghae OGEFERE University of Benin, College of Medical Sciences, School of Basic Medical Sciences, Department of Medical Laboratory Science, Benin City, Nigeria
  • Salome O. AGBE University of Benin, College of Medical Sciences, School of Basic Medical Sciences, Department of Medical Laboratory Science, Benin City, Nigeria
  • Ephraim Ehidiamen IBADIN Medical Microbiology Unit, Medical Laboratory Services, University of Benin Teaching Hospital, Benin City, Nigeria

Abstract

This study was carried out to determine the prevalence of extended spectrum beta-lactamase (ESBL) among Gram negative bacteria isolated from cattle feces in Benin City, Nigeria. A total of 250 Gram negative bacteria isolates were recovered from cattle feces and were processed microbiologically using standard techniques. Emergent colonies were identified and antibacterial susceptibility tests were determined using Kirby-Bauer disk diffusion method. All bacterial isolates were screened for the presence of ESBL using the double-disc synergy method. A total of 37 (14.8%) isolates were positive for ESBL, with 33 (13.2%) indicated by ceftazidime, while only 4 (1.6%) were indicated by both ceftazidime and cefotaxime (P < 0.0001). Of the Gram negative bacterial isolates recovered, Salmonella species was the most prevalent ESBL-producer with 55.0% prevalence (P = 0.0092), while no isolate of Pseudomonas aeruginosa produced ESBL. ESBL-positive isolates showed poor susceptibility to the tested antibacterial agents in comparison with non-ESBL-producers and imipenem was the most active antibiotic. The prevalence of ESBL among Gram negative bacilli recovered from cattle feces was 14.8%. The study advises prudent use of antibiotics in the treatment of cattle and harps on improved hygiene in managing cattle, as they are potential reservoirs of ESBL-producing organisms.

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Author Biographies

Helen Oroboghae OGEFERE, University of Benin, College of Medical Sciences, School of Basic Medical Sciences, Department of Medical Laboratory Science, Benin City, Nigeria
Senior Lecturer, Department of Medical Laboratory Science, 

School of Basic Medical Sciences,

College of Medical Sciences,

University of Benin,

Benin City, Nigeria.

 

Salome O. AGBE, University of Benin, College of Medical Sciences, School of Basic Medical Sciences, Department of Medical Laboratory Science, Benin City, Nigeria

Student, 

Department of Medical Laboratory Science, 

School of Basic Medical Sciences,

College of Medical Sciences,

University of Benin,

Benin City, Nigeria.

 

Ephraim Ehidiamen IBADIN, Medical Microbiology Unit, Medical Laboratory Services, University of Benin Teaching Hospital, Benin City, Nigeria
Medical Laboratory Scientist
Published
2017-06-30
How to Cite
OGEFERE, H. O., AGBE, S. O., & IBADIN, E. E. (2017). Detection of Extended Spectrum Beta-Lactamases Among Gram Negative Bacilli Recovered from Cattle Feces In Benin City, Nigeria. NOTULAE SCIENTIA BIOLOGICAE, 9(2), 177-181. https://doi.org/10.15835/nsb9210005
Section
Research articles